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Published on: July 17, 2012
Application of an indocyanine green-mediated fluorescence imaging navigation system in detecting mice tumors
Xueyan Zhao1, Ziyu Wang1, Yulin Wu2
1School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China.
Background:
Surgical operation plays an important role in the treatment of cancer. The success of the operation lies in the complete removal of the primary and disseminated tumor tissue while preserving the normal tissue. The development of optical molecular image navigation technology has provided a new option for intraoperative tumor visualization. In this study, a fluorescence imaging navigation system was used to detect the diameter of mice tumors and provide experimental evidence for the further development of digital diagnosis and treatment equipment.
Methods:
The minimum detection concentration in vitro of the fluorescence imaging navigation system for indocyanine green (ICG) was first detected, then 120 female Institute of Cancer Research (ICR) mice and 120 female BALB/c nude mice were randomly divided into three groups by weight, high-dose (H, 4 mg/kg), middle-dose (M, 2 mg/kg), and low-dose (L, 1 mg/kg) groups of ICG solution. After inoculating solid tumors, high, medium, and low doses of ICG were injected via the tail vein, and the tumor diameter was measured by a fluorescence imaging navigation system and vernier caliper within 24 hours of injection.
Results:
The minimum detectable diameter of the system could reach 0.2 mm compared with the vernier caliper, and the actual measurement error was within 0.2 mm.
Conclusions:
A fluorescence imaging navigation system has high accuracy and sensitivity in the application of tumor detection, which may assist the clinical diagnosis and treatment of tumors.
Insights
This study demonstrates a fluorescence imaging navigation system accurately detects small mouse tumors, with a minimum detectable diameter of 0.2 mm. This technology shows promise for improving intraoperative tumor visualization and aiding clinical cancer diagnosis and treatment.
Area of Science:
- Medical Imaging
- Surgical Navigation
- Oncology
Background:
- Surgical success in cancer treatment relies on complete tumor removal while preserving healthy tissue.
- Optical molecular imaging offers new possibilities for intraoperative tumor visualization.
- This study evaluated a fluorescence imaging navigation system for tumor detection.
Purpose of the Study:
- To assess the accuracy and sensitivity of a fluorescence imaging navigation system for detecting mouse tumors.
- To provide experimental data for the development of digital diagnosis and treatment equipment.
Main Methods:
- Determined the minimum detection concentration of indocyanine green (ICG) in vitro.
- Administered varying doses of ICG to mice with solid tumors via tail vein injection.
- Measured tumor diameters using the fluorescence imaging system and a vernier caliper within 24 hours.
Main Results:
- The fluorescence imaging system detected tumors with a minimum diameter of 0.2 mm.
- The measurement error of the system was within 0.2 mm when compared to a vernier caliper.
Conclusions:
- The fluorescence imaging navigation system exhibits high accuracy and sensitivity for tumor detection.
- This technology has the potential to assist in the clinical diagnosis and treatment of tumors.
