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Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...

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Updated: Oct 19, 2025

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research

Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research

Published on: April 18, 2013

Multiplexed all-solid-state ion-sensitive light-addressable potentiometric sensor (ISLAPS) system based on

Tao Liang¹, Nan Jiang¹, Shuqi Zhou¹

¹Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.

Analytica Chimica Acta

|September 18, 2021

Summary

Researchers developed a novel multiplexed ion-sensitive light-addressable potentiometric sensor (ISLAPS) system for detecting physiological ions like Na+, K+, Ca2+, and H+. This all-solid-state system utilizes silicone-rubber membranes for improved stability and avoids plasticizer issues, offering a promising tool for multiparameter analysis.

Keywords:

All-solid-state ion-sensitive membrane (ISM)Light-addressable potentiometric sensor (LAPS)Multi-parameter sensor system Physiological ions detection Silicone-rubber membrane

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Area of Science:

Biomedical Engineering
Sensor Technology
Analytical Chemistry

Background:

Light-addressable potentiometric sensors (LAPS) are vital in biomedical applications.
Conventional ion-selective electrodes (ISEs) face limitations like maintenance and miniaturization challenges.
Existing ion-sensitive membrane (ISM) matrices, such as PVC, exhibit poor adhesion and plasticizer leakage issues.

Purpose of the Study:

To develop a program-controlled multiplexed ion-sensitive LAPS (ISLAPS) system for simultaneous detection of physiological ions.
To overcome limitations of traditional ISEs and PVC-based ISMs by employing all-solid-state silicone-rubber ISMs.
To enhance sensor performance through improved adhesion, plasticizer-free membranes, and pre-modification with poly(3-octylthiophene-2,5-diyl) (P3OT).

Main Methods:

Integration of LAPS with ionophore-doped all-solid-state silicone-rubber ISMs for Na+, K+, Ca2+, and H+ detection.
Pre-modification of the sensor surface with P3OT to prevent aqueous layer formation and extend sensor lifetime.
Automated sequential illumination of detection sites using a translation stage for rapid, multiplexed measurements.

Main Results:

The developed multiplexed ISLAPS system demonstrated good sensitivity with a micromolar limit of detection (LOD).
The system exhibited excellent selectivity and long-term stability, maintaining performance for over 3 months.
Successful detection of physiological ions in real Dulbecco's Modified Eagle Medium (DMEM) samples was achieved.

Conclusions:

The novel all-solid-state silicone-rubber ISLAPS system offers a stable and reliable platform for physiological ion detection.
The multiplexed approach enables rapid, simultaneous measurement of multiple ions, overcoming previous limitations.
This technology shows significant promise for developing advanced multi-parameter microphysiometers for biomedical research.