Sterol Analysis in Kluyveromyces lactis.

Area of Science:

• Biochemistry
• Molecular Biology
• Mycology

Background:

• Sterols are vital eukaryotic lipids with structural, regulatory, and developmental roles.
• Yeast sterol profiles are studied using ergosterol synthesis mutants to understand sterol functions.
• Ergosterol biosynthesis pathways are targets for antifungal drug development.

Purpose of the Study:

• To present a protocol for isolating non-saponifiable lipids (sterols) from *Kluyveromyces lactis*.
• To describe a High-Performance Liquid Chromatography (HPLC) method for quantitative sterol analysis.
• To provide a method adaptable for standard laboratory equipment.

Main Methods:

• Isolation of non-saponifiable lipids from *Kluyveromyces lactis* yeast cells.
• Quantitative analysis of sterols using High-Performance Liquid Chromatography (HPLC).

Main Results:

• A reproducible protocol for sterol isolation from yeast.
• An effective HPLC method for sterol quantification in lipid extracts.

Conclusions:

• The presented protocol enables efficient sterol isolation and analysis in yeast.
• This method supports research into sterol functions and antifungal development.