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Isolation and Quantification of Plant Extracellular Vesicles
Brian D Rutter1, Katie L Rutter1, Roger W Innes1
1Department of Biology, Indiana University, Bloomington, IN, USA.
Bio-Protocol
|September 20, 2021
Summary
Researchers developed a method to isolate plant extracellular vesicles (EVs) from Arabidopsis leaves. This protocol allows for the quantification of these important intercellular communication vesicles.
Area of Science:
- Plant biology
- Cell biology
- Biochemistry
Background:
- Extracellular vesicles (EVs) are crucial for intercellular communication, transporting proteins and RNA.
- Plant EVs are recently discovered, with limited understanding of their biogenesis, release, uptake, and function.
Purpose of the Study:
- To present a detailed protocol for isolating EVs from the apoplastic wash of Arabidopsis thaliana leaves.
- To enable quantification of isolated plant EVs.
Main Methods:
- Isolation of EVs from the apoplastic wash of Arabidopsis thaliana leaves.
- Quantification of isolated EVs using a fluorometric dye to assess total membrane content.
Main Results:
- A detailed protocol for isolating plant EVs was successfully established.
- The method allows for the quantification of isolated EVs based on membrane content.
Conclusions:
- The presented protocol facilitates the study of plant EVs.
- This method is a key step towards understanding plant intercellular communication via EVs.
