Related Experiment Video
Updated: Oct 19, 2025
11:14
Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
Published on: November 7, 2018
18.5K
RNA Strand Displacement Assay for Hepatitis E Virus Helicase
1Virology Laboratory, Vaccine and Infectious Disease Research Centre, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, Haryana, India.
Bio-Protocol
|September 20, 2021
Summary
Hepatitis E virus (HEV) helicase unwinds double-stranded RNA using ATP, a crucial step for viral replication. This protocol details how to measure this essential HEV helicase activity.
Area of Science:
- Virology
- Molecular Biology
- Biochemistry
Background:
- Hepatitis E virus (HEV) is a significant human pathogen.
- Viral replication relies on essential viral enzymes like helicases.
- HEV helicase activity is critical for its life cycle.
Purpose of the Study:
- To establish a protocol for measuring the double-strand RNA unwinding activity of the HEV helicase.
- To provide a method for biochemical characterization of HEV helicase function.
Main Methods:
- The protocol involves measuring the ATP-dependent unwinding of RNA duplexes by the HEV helicase.
- Specific biochemical assays are employed to quantify unwinding efficiency.
Main Results:
- The protocol successfully measures the double-strand RNA unwinding activity of the HEV helicase.
- Data on HEV helicase's ATP-dependent unwinding capability can be obtained.
Conclusions:
- This protocol provides a reliable method for assessing a key enzymatic activity of the HEV helicase.
- Understanding HEV helicase function is vital for developing antiviral strategies against Hepatitis E.
