Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila for Negative Stain
1Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Bio-Protocol
|September 20, 2021
Abstract:
Legionella possesses a pivotal secretion machinery to deliver virulence factors to eukaryotic host cells. In this protocol, we describe the procedure for isolation of the native core complex of the Dot/Icm type IV secretion system from L. pneumophila aiming to perform biochemical and transmission electron microscopy analyses.
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