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Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos
Published on: June 26, 2017
Dimming the donor to brighten up FRET-based biosensors
Ji Jing1, Yubin Zhou2, Youjun Wang3
1The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer, Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.
Abstract:
The current biosensors based on Förster resonance energy transfer (FRET) lack good anisotrophy-based indicators for detecting rotational changes triggered by analyte-binding. Laskaratou et al. developed a FRET-induced Angular Displacement Evaluation via Dim donor (FADED) tool to expand the existing toolkit. With less bleed-through from a donor with dim fluorescence, the changes in acceptor anisotropy signals of these tools could readily indicate cytosolic calcium dynamics.

