Antibacterial activity of ZnO quantum dots and its protective effects of chicks infected withSalmonella pullorum

In light of emerging antibiotic resistance, synthesis of active, environmental friendly antimicrobial alternatives becomes increasingly necessary. In this study, ZnO quantum dots (ZnO QDs) were developed by the sol-gel method and characterized. The antibacterial activities of ZnO QDs againstEscherichia coli(E. coli),Staphylococcus aureus(S. aureus) andSalmonella Pullorum(S. Pullorum) were systematically investigated. Moreover, the protective effects of ZnO QDs on Salmonella-caused pullorosis in chicks were also explored. The results indicated that the size range of ZnO QDs was 3-6 nm. Antibacterial results showed that ZnO QDs treatment inhibited the growth ofE. coli,S. aureus, andS. Pullorumin the rate of 87.06 ± 0.98%, 94.75 ± 2.28%, and 85.55 ± 1.15%, respectively. Its excellent antibacterial property was manifested with the minimum inhibitory concentration of 0.7812, 0.0976, and 0.1953 mg ml^-1, which may be attributed to the production of reactive oxygen species, the dissolution of Zn²⁺ions, and the loss of cell integrity. Furthermore, in thein vivotest, the ZnO QDs effectively reduced the mortality of chicks infected withS. Pullorumvia regulating the balance of the intestinal flora, protecting liver and intestine, and modulating the balance of antioxidation systems. This study reveals that ZnO QDs exerts remarkably antibacterial activityin vitroand anti-pullorosis effect in chicks.