Chenopodin as an anti-inflammatory compound

Area of Science:

• Biochemistry
• Immunology
• Pharmacology

Background:

• Chenopodin is an 11S-type globulin isolated from *Chenopodium quinoa* seeds.
• It possesses carbohydrate-binding and hemagglutinating properties.
• The N-terminal structure and anti-inflammatory potential of Chenopodin were previously uncharacterized.

Purpose of the Study:

• To elucidate the N-terminal structure of heterodimeric Chenopodin.
• To investigate the *in vivo* anti-inflammatory effects of Chenopodin.
• To determine Chenopodin's impact on edema and neutrophil recruitment.

Main Methods:

• Purification of Chenopodin from *Chenopodium quinoa* seeds.
• Analysis of the N-terminal structure and subunit composition.
• Assessment of anti-inflammatory activity using a carrageenan-induced paw edema model in mice.
• Quantification of neutrophil recruitment.

Main Results:

• Chenopodin comprises two subunits with 92% N-terminal homology.
• Administration of Chenopodin significantly reduced carrageenan-induced paw edema in mice.
• Chenopodin treatment markedly decreased neutrophil infiltration into inflamed tissue.

Conclusions:

• Chenopodin exhibits significant *in vivo* anti-inflammatory activity.
• The protein effectively modulates key inflammatory responses, including edema and neutrophil recruitment.
• Chenopodin represents a potential therapeutic agent for inflammatory conditions.