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Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Huy Cuong Tran1, Olivier Van Aken2

  • 1Department of Biology, Lund University, Lund, Sweden.

Methods in Molecular Biology (Clifton, N.J.)
|September 21, 2021
PubMed

Keywords:
ArabidopsisDensity gradientsMitochondriaMitochondrial isolation

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Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...