The protein kinase SlCIPK23 boosts K⁺ and Na⁺ uptake in tomato plants

Regulation of root transport systems is essential under fluctuating nutrient supply. In the case of potassium (K⁺ ), HAK/KUP/KT K⁺ transporters and voltage-gated K⁺ channels ensure root K⁺ uptake in a wide range of K⁺ concentrations. In Arabidopsis, the CIPK23/CBL1-9 complex regulates both transporter- and channel-mediated root K⁺ uptake. However, research about K⁺ homeostasis in crops is in demand due to species-specific mechanisms. In the present manuscript, we studied the contribution of the voltage-gated K⁺ channel LKT1 and the protein kinase SlCIPK23 to K⁺ uptake in tomato plants by analysing gene-edited knockout tomato mutant lines, together with two-electrode voltage-clamp experiments in Xenopus oocytes and protein-protein interaction analyses. It is shown that LKT1 is a crucial player in tomato K⁺ nutrition by contributing approximately 50% to root K⁺ uptake under K⁺ -sufficient conditions. Moreover, SlCIPK23 was responsible for approximately 100% of LKT1 and approximately 40% of the SlHAK5 K⁺ transporter activity in planta. Mg⁺² and Na⁺ compensated for K⁺ deficit in tomato roots to a large extent, and the accumulation of Na⁺ was strongly dependent on SlCIPK23 function. The role of CIPK23 in Na⁺ accumulation in tomato roots was not conserved in Arabidopsis, which expands the current set of CIPK23-like protein functions in plants.