Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Electron Microscopy

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Phasor EO-FLIM: Lifetime imaging with picosecond noise and 500 Hz frame rate.

bioRxiv : the preprint server for biology·2026

Same author

Fast wide-field light sheet electro-optic FLIM.

Optics express·2026

Same author

Wide-field fluorescence lifetime imaging of neuron spiking and subthreshold activity in vivo.

Science (New York, N.Y.)·2023

Same author

Distributed quantum sensing with mode-entangled spin-squeezed atomic states.

Nature·2022

Same author

Observation of a gravitational Aharonov-Bohm effect.

Science (New York, N.Y.)·2022

Same author

Fast pulse shaping for a novel gated electron mirror.

The Review of scientific instruments·2021

Same journal

Nongenetic <i>in Vivo</i> Bimodal Neuromodulation via Photothermal Gold Nanorods and a Multifunctional Fiber Neural Probe.

ACS nano·2026

Same journal

Electric-Field-Driven Ferredoxin 1-Independent Cuproptosis Induction Overcomes Therapy-Induced Resistance in Glioblastoma.

ACS nano·2026

Same journal

Connecting and Engaging.

ACS nano·2026

Same journal

Efficient Photocatalytic Methane Conversion to Liquid Oxygenates by Constructing Charge-Directed Transfer Pathways.

ACS nano·2026

Same journal

Mechanochemically Coupled Multidimensional Modulation of Calcium Overload.

ACS nano·2026

Same journal

Electrical Control and High-Bias Enhancement of Magnetoresistance in van der Waals Antiferromagnetic Spin-Filter Tunnel Field-Effect Transistor.

ACS nano·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 19, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Resonant Electro-Optic Imaging for Microscopy at Nanosecond Resolution.

Adam J Bowman¹, Mark A Kasevich¹

¹Physics Department, Stanford University, 382 Via Pueblo Mall, Stanford, California 94305, United States.

|September 21, 2021

Summary

This summary is machine-generated.

We developed a fast electro-optic method for fluorescence lifetime microscopy (FLIM) enabling high-throughput, single-molecule imaging. This technique captures fast molecular dynamics in wide fields with millisecond exposures, advancing microscopy capabilities.

Keywords:

FLIM Pockels cell electro-optic nanosecond imaging single-molecule super-resolution wide-field

More Related Videos

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Published on: August 26, 2010

Related Experiment Videos

Last Updated: Oct 19, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Optical Recording of Suprathreshold Neural Activity with Single-cell and Single-spike Resolution

Published on: September 5, 2012

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Published on: August 26, 2010

Area of Science:

Biophysics
Optical Microscopy
Spectroscopy

Background:

Fluorescence Lifetime Imaging Microscopy (FLIM) is crucial for analyzing molecular dynamics.
Current FLIM methods often lack high throughput or single-molecule sensitivity.
Integrating FLIM with super-resolution microscopy presents technical challenges.

Purpose of the Study:

To develop a high-throughput, single-molecule sensitive wide-field fluorescence lifetime microscopy method.
To enable capture of fast single-molecule dynamics using FLIM.
To combine FLIM with super-resolution localization microscopy.

Main Methods:

Utilized resonantly driven Pockels cells for efficient image gating at 39 MHz.
Employed standard camera sensors for fluorescence lifetime capture.
Achieved millisecond exposure times for wide-field FLIM acquisition.

Main Results:

Demonstrated single-molecule FLIM in wide field with <100 ms exposure times.
Achieved a lifetime sensitivity of 1.9 times the photon shot-noise limit.
Acquired wide-field FLIM images with >10^8 photons per frame, showcasing high throughput.

Conclusions:

The resonant electro-optic FLIM method provides high throughput and single-molecule sensitivity.
This technique allows capturing fast dynamics like FRET and binding events without prior spatial knowledge.
Electro-optic FLIM can be integrated into various wide-field microscopy techniques for enhanced contrast.