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Element probe based CRISPR/Cas14 bioassay for non-nucleic-acid targets
Jianyu Hu1, Jing Zhou1, Rui Liu2
1Analytical & Testing Center, Sichuan University, Chengdu 610064, P. R. China. lvy@scu.edu.cn.
Chemical Communications (Cambridge, England)
|September 22, 2021
Summary
A new CRISPR/Cas14 detection platform uses element probes for sensitive, rapid detection of non-nucleic targets like ampicillin. This biosensing method achieves low detection limits in complex samples.
Area of Science:
- Biotechnology
- Biosensing
- Molecular Biology
Background:
- CRISPR/Cas systems are primarily known for nucleic acid detection and editing.
- Detecting non-nucleic acid targets with high sensitivity remains a challenge in various fields.
Purpose of the Study:
- To develop a novel CRISPR/Cas14-based detection platform for non-nucleic acid targets.
- To combine metal isotope detection with CRISPR/Cas14 biosensing for enhanced sensitivity.
- To optimize element probe design for Cas14 activity.
Main Methods:
- Design and optimization of element probes for CRISPR/Cas14.
- Integration of metal isotope detection with CRISPR/Cas14 biosensing.
- Application of the platform for quantitative detection of ampicillin in aqueous solutions.
Main Results:
- Cas14 demonstrated a preference for longer element probes during cleavage.
- Quantitative detection of trace ampicillin achieved within 45 minutes at room temperature.
- A low detection limit of 2.06 nM was obtained.
- The method showed excellent anti-interference capabilities and performance in complex matrices.
Conclusions:
- The element probe-based CRISPR/Cas14 platform enables sensitive detection of non-nucleic acid targets.
- This approach offers a rapid, room-temperature method for quantitative analysis.
- The platform shows promise for detecting trace contaminants in complex samples.
