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Circular RNA WHSC1 exerts oncogenic properties by regulating miR-7/TAB2 in lung cancer

Sisi Guan1, Li Li1, Wen-Shu Chen2

  • 1Department of Geriatrics, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Journal of Cellular and Molecular Medicine
|September 22, 2021
PubMed

Insights

Circular RNA circ-WHSC1 promotes non-small cell lung cancer (NSCLC) progression by regulating miR-7 and TAB2. Targeting this circ-WHSC1/miR-7/TAB2 pathway may offer a new therapeutic strategy for NSCLC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are novel non-coding RNAs regulating gene expression.
  • circRNAs function as micro-RNA sponges, impacting various diseases.
  • The role of circRNAs in non-small cell lung cancer (NSCLC) is not fully understood.

Purpose of the Study:

  • To investigate the function of circ-WHSC1 in NSCLC.
  • To elucidate the molecular mechanism of circ-WHSC1 in NSCLC progression.
  • To evaluate the circ-WHSC1/miR-7/TAB2 axis as a potential therapeutic target in NSCLC.

Main Methods:

  • Quantitative real-time PCR to assess circ-WHSC1 expression in NSCLC tissues and cells.
  • In vitro assays (proliferation, migration, invasion) and in vivo xenograft models to evaluate circ-WHSC1's functional role.
  • Bioinformatic analysis, luciferase reporter assays, and Western blotting to identify and validate the interaction between circ-WHSC1, miR-7, and TAB2.

Main Results:

  • circ-WHSC1 was significantly upregulated in NSCLC tissues and cells.
  • circ-WHSC1 overexpression promoted NSCLC cell proliferation, migration, and invasion.
  • circ-WHSC1 acted as a sponge for miR-7, thereby upregulating TAB2 expression.
  • Inhibition of the circ-WHSC1/miR-7/TAB2 pathway suppressed NSCLC progression.

Conclusions:

  • circ-WHSC1 exhibits oncogenic functions in NSCLC.
  • The circ-WHSC1/miR-7/TAB2 signaling pathway plays a critical role in NSCLC development.
  • circ-WHSC1 represents a potential diagnostic biomarker and therapeutic target for NSCLC.
Keywords:
TAB2circ-WHSC1miR-7non-small cell lung cancer

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