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Tissue and sperm handling before assisted reproductive technology (ART): A systematic review
Rafael Favero Ambar1,2, Marcello M Gava1,2, Milton Ghirelli-Filho1,2
1Urology Department at Centro Universitario Em Saude Do ABC - FMABC, Santo André, Brazil.
Objective:
: To explore the medical literature on techniques of tissue and sperm handling after surgical retrieval for intracytoplasmic sperm injection (ICSI).
Methods:
: A search was performed in PubMed and Google Scholar databases, according to a modified Preferred Reporting Items for Systemic Reviews and Meta-Analyses (PRISMA) guideline, considering the studies investigating tissue handling and sperm selection techniques for ICSI.
Results:
: Overall, 42 articles were included in this study, investigating sample handling, methods for sperm selection, and the use of chemical compounds to improve sperm motility and fertilisation rates.
Conclusion:
: The ideal sperm handling method should provide a high sperm count, high vitality and appropriate sperm function, without side-effects. In this review the most common and useful techniques are described and the best combination strategies discussed in clinical scenarios.
