A sensitive biosensor for glucose determination based on the unique catalytic chemiluminescence of sodium molybdate

Chemiluminescent (CL) reaction between hydrogen peroxide (H₂O₂) and luminol was dramatically enhanced by sodium molybdate (Na₂MoO₄) for 284-fold. CL mechanism investigation indicated that Na₂MoO₄ increased the production of hydroxyl radical (•OH) and superoxide anion (•O₂^-) in the H₂O₂-luminol system, which could attribute to the enhanced-CL intensity and gave us new insights into the CL-enhanced property of Na₂MoO₄. The CL intensity of Na₂MoO₄-H₂O₂-luminol system increased with the concentration of H₂O₂, based on which, a convenient and sensitive CL determination method could be developed for H₂O₂ in the concentration ranging from 0.5 to 60 μmol/L, with a detection limit of 0.25 μmol/L. Combining with glucose oxidase, the Na₂MoO₄-H₂O₂-luminol system could also be applied for glucose detection. Glucose in human serum has been successfully detected with satisfied recoveries in the range of 96.7 % to 105.4 %.