Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

13.1K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
13.1K
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

3.9K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.9K
Pneumothorax-I01:26

Pneumothorax-I

608
A pneumothorax is a condition where air builds up in the space between the lung and the chest wall, causing the lung to collapse. This condition arises when air enters the space between the parietal and visceral pleura, disrupting the negative pressure essential for lung inflation. This can lead to a partial or complete collapse of the lung.
Pneumothorax can be even further classified as spontaneous, traumatic, and tension pneumothorax.
608
The Electron Transport Chain01:30

The Electron Transport Chain

18.1K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
18.1K
Breathing01:05

Breathing

60.9K
The process of breathing, inhaling and exhaling, involves the coordinated movement of the chest wall, the lungs, and the muscles that move them. Two muscle groups with important roles in breathing are the diaphragm, located directly below the lungs, and the intercostal muscles, which lie between the ribs. When the diaphragm contracts, it moves downward, increasing the volume of the thoracic cavity and creating more room for the lungs to expand. When the intercostal muscles contract, the ribs...
60.9K
Mitochondria01:37

Mitochondria

15.8K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
15.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Incremental contribution of bronchoalveolar lavage fluid data to multidisciplinary discussion of interstitial lung disease.

BMC pulmonary medicine·2026
Same author

High blood pressure and associated metabolic disorders among young adults with and without perinatal HIV.

AIDS (London, England)·2026
Same author

Pulmonary Arterial Hypertension and Endothelial Dysfunction.

Handbook of experimental pharmacology·2026
Same author

Aging and cardiopulmonary interactions: physiologic and pathophysiologic consequences.

American journal of physiology. Heart and circulatory physiology·2026
Same author

MSC-EVs attenuate subretinal fibrosis in choroidal neovascularization through miR-21-5p-mediated inhibition of EMT and MMT and suppression of inflammation.

Journal of neuroinflammation·2026
Same author

Defining the Resolution of Acute Respiratory Distress Syndrome: A Missing Piece in Critical Care.

Critical care medicine·2026

Related Experiment Video

Updated: Oct 19, 2025

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells
08:33

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells

Published on: February 8, 2017

41.9K

Sepsis Disrupts Mitochondrial Function and Diaphragm Morphology.

Thamires Siqueira Oliveira1, Anderson Teixeira Santos2, Cherley Borba Vieira Andrade1

  • 1Laboratory of Translational Endocrinology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

Frontiers in Physiology
|September 24, 2021
PubMed
Summary

Sepsis causes diaphragm muscle damage, including mitochondrial dysfunction and impaired function. This study reveals significant morphophysiological changes in the diaphragm during sepsis, highlighting mitochondrial damage as a key factor.

Keywords:
CLPdiaphragmmitochondriamuscleoxidative phosphorylationsepsis

More Related Videos

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis
04:01

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis

Published on: June 14, 2024

1.0K
A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
08:46

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

Published on: August 12, 2020

6.5K

Related Experiment Videos

Last Updated: Oct 19, 2025

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells
08:33

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells

Published on: February 8, 2017

41.9K
Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis
04:01

Author Spotlight: Advanced Integrated Model for Sepsis-Induced Myopathy and Single-Cell Metabolic Analysis

Published on: June 14, 2024

1.0K
A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis
08:46

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

Published on: August 12, 2020

6.5K

Area of Science:

  • Physiology
  • Pathology
  • Mitochondrial Biology

Background:

  • Diaphragm dysfunction is common in sepsis but poorly understood.
  • Sepsis significantly impacts respiratory muscle function.
  • Mechanisms linking sepsis to diaphragm impairment require elucidation.

Purpose of the Study:

  • To investigate the morphophysiological changes in the diaphragm mitochondria during sepsis.
  • To evaluate the functional consequences of sepsis on diaphragm muscle.
  • To identify molecular alterations in diaphragm muscle following sepsis induction.

Main Methods:

  • Cecal ligation and puncture (CLP) model in mice to induce sepsis.
  • Diaphragm function assessed via *in vivo* ultrasound.
  • Myosin heavy chain, SERCA, Myod1, and Myog gene and protein expression analyzed.
  • Mitochondrial ultrastructure, physiology, and respiratory function evaluated using electron microscopy and high-resolution respirometry.
  • Mitochondrial dynamics markers (PGC1a, OPA1) and antioxidant enzyme (Sod2) expression quantified.

Main Results:

  • Sepsis induced sarcomeric disorganization and increased diaphragm thickness without altering mass.
  • Significant reduction in mitochondrial content and respiratory chain complex expression observed.
  • Impaired mitochondrial oxygen consumption and increased proton leak noted in septic diaphragms.
  • Downregulation of key mitochondrial biogenesis (PGC1a) and fusion (OPA1) markers, alongside increased non-functional OPA1.

Conclusions:

  • Sepsis induces significant morphological and functional impairments in the diaphragm.
  • Mitochondrial damage and dysfunction are central to sepsis-induced diaphragm pathology.
  • Findings suggest therapeutic targets for mitigating diaphragm dysfunction in sepsis.