Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

10.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
10.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Theoretical Study on the Quantum Dynamics of Highly Energetic <sup>14</sup>N + <sup>15</sup>N<sup>15</sup>N Isotope Exchange Reactions.

The journal of physical chemistry. A·2026
Same journal

Reaction-Coordinate Analysis of DMAP-Mediated Allylation of Allylic Alcohols: Coupled Proton Transfer and C-C Bond Formation.

The journal of physical chemistry. A·2026
Same journal

Oligoterpenes Oxidation: Integrating Thermal Analysis Experiments and <i>Ab Initio</i> Kinetics.

The journal of physical chemistry. A·2026
Same journal

URVAvis: A Graphical User Interface for the Visual Mechanistic Analysis of Chemical Reactions Based on the Unified Reaction Valley Approach.

The journal of physical chemistry. A·2026
Same journal

Magnetic Exchange and Electronic Structure across a Series of NHC-Stabilized Boron Diradicals.

The journal of physical chemistry. A·2026
Same journal

Effect of Fluorination on Excited-State Dynamics in Methyl Salicylate: A Surface-Hopping Dynamics Study.

The journal of physical chemistry. A·2026

Related Experiment Video

Updated: Oct 19, 2025

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

10.5K

High Sensitivity Frequency Modulation Spectroscopy and the Path to Single Molecule Detection.

Gary C Bjorklund1, Edward A Whittaker2

  • 1Bjorklund Enterprises, POB 1420, Pebble Beach, California 93953, United States.

The Journal of Physical Chemistry. A
|September 24, 2021
PubMed
Summary

Frequency modulation laser spectroscopy (FMS) advances detection limits in optical spectroscopy. Double modulation FMS enabled the sensitive measurement of single-molecule absorption, a key step in single-molecule spectroscopy.

More Related Videos

Single-Molecule F&#246;rster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

9.6K
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.2K

Related Experiment Videos

Last Updated: Oct 19, 2025

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
09:48

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Published on: February 27, 2015

10.5K
Single-Molecule F&#246;rster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

9.6K
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
08:27

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Published on: October 1, 2016

9.2K

Area of Science:

  • Optical Spectroscopy
  • Quantum Optics
  • Analytical Chemistry

Background:

  • Advances in optical spectroscopy depend on noise reduction to fundamental detection limits.
  • Frequency modulation laser spectroscopy (FMS) approaches the quantum limit for detection.
  • Double modulation FMS has demonstrated high sensitivity in absorption spectroscopy.

Purpose of the Study:

  • To trace the development of FMS from its initial demonstration to its application in single-molecule detection.
  • To emphasize the role of double modulation methods in achieving high sensitivity for measuring small absorptions.
  • To highlight the significance of FMS in the advancement of single-molecule spectroscopy.

Main Methods:

  • Review of the historical development of frequency modulation laser spectroscopy (FMS).
  • Focus on double modulation techniques within FMS.
  • Examination of key milestones, including the first single-molecule detection by Moerner and Kador.

Main Results:

  • FMS, particularly double modulation FMS, has significantly improved detection sensitivity in optical spectroscopy.
  • The development of FMS paved the way for the optical detection and probing of single dopant molecules in solids.
  • This progress enabled the field of single-molecule spectroscopy.

Conclusions:

  • Double modulation FMS is crucial for achieving the sensitivity required for single-molecule absorption measurements.
  • The evolution of FMS has been instrumental in pushing the boundaries of optical detection.
  • FMS continues to be a vital technique for high-sensitivity spectroscopic measurements.