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Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
Published on: June 30, 2023
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Bacillus spore enumeration using flow cytometry: A proof of concept for probiotic application
Maxime Genovese1, Edith Poulain1, Florie Doppler1
1Research and Development Department, Lesaffre International, Lesaffre Group, 59700 Marcq-en-Baroeul, France.
Journal of Microbiological Methods
|September 24, 2021
Abstract:
Use of flow cytometry (FCM) for bacteria quantification is growing in the food industry. We report here a FCM method using a double-staining LDS751/SYTO24 for the quantification of probiotic Bacillus viable cells and its spores, with potential application for the control of commercial product specifications.

