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Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry
Published on: June 30, 2023
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Bacillus spore enumeration using flow cytometry: A proof of concept for probiotic application.
Maxime Genovese1, Edith Poulain1, Florie Doppler1
1Research and Development Department, Lesaffre International, Lesaffre Group, 59700 Marcq-en-Baroeul, France.
Journal of Microbiological Methods
|September 24, 2021
Summary
A new flow cytometry method uses double-staining for accurate quantification of probiotic Bacillus cells and spores. This technique aids in controlling commercial product specifications in the food industry.
Area of Science:
- Microbiology
- Food Science
- Analytical Chemistry
Background:
- Flow cytometry (FCM) is increasingly utilized for bacterial quantification in the food sector.
- Accurate enumeration of viable cells and spores is crucial for probiotic product quality.
Purpose of the Study:
- To develop and validate a novel FCM method for quantifying probiotic Bacillus viable cells and spores.
- To assess the method's applicability in commercial product specification control.
Main Methods:
- Utilized a flow cytometry technique employing a double-staining combination of LDS751 and SYTO24 dyes.
- Applied the method for the enumeration of both viable Bacillus cells and their corresponding spores.
Main Results:
- The developed FCM method successfully quantified probiotic Bacillus viable cells and spores.
- The double-staining approach provided reliable enumeration data.
Conclusions:
- The novel FCM method offers a robust tool for the precise quantification of Bacillus viable cells and spores.
- This technique has significant potential for ensuring quality control in probiotic food products.
