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Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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Screening for differentially expressed circRNAs in ischemic stroke by RNA sequencing
Duncan Wei1, Jian Chen2, Xiaopu Chen3
1Department of Pharmacy, The First Affiliated Hospital of Shantou University Medical College, No. 57, Changping Road, Guangdong, 515041, Shantou, China.
BMC Neurology
|September 26, 2021
Summary
This study identified 736 differentially expressed circRNAs (DEcircRNAs) in ischemic stroke patients, revealing potential diagnostic biomarkers like hsa_circ_0000745. These findings offer insights into the molecular mechanisms of ischemic stroke.
Area of Science:
- Biochemistry
- Genetics
- Molecular Biology
Background:
- Ischemic stroke is a leading cause of death and disability globally.
- Circular RNAs (circRNAs) are novel non-coding RNAs implicated in ischemic stroke pathogenesis.
- Understanding circRNA roles is crucial for developing new therapeutic strategies.
Purpose of the Study:
- To identify key differentially expressed circRNAs (DEcircRNAs) associated with ischemic stroke.
- To explore the functional pathways and molecular interactions of these DEcircRNAs.
- To evaluate the diagnostic potential of specific circRNAs in ischemic stroke.
Main Methods:
- RNA sequencing was employed to profile circRNA expression in ischemic stroke patients and healthy controls.
- Bioinformatic analyses identified DEcircRNAs and enriched functional pathways.
- Quantitative reverse transcription PCR (qRT-PCR) validated circRNA expression.
- A circRNA-miRNA interaction network was constructed to elucidate regulatory mechanisms.
Main Results:
- A total of 736 DEcircRNAs were identified in ischemic stroke.
- Enriched pathways included Fc epsilon RI, B cell receptor, and T cell receptor signaling.
- Four specific circRNAs (hsa_circ_0000745, hsa_circ_0001459, hsa_circ_0003694, hsa_circ_0007706) showed high diagnostic value.
- A comprehensive circRNA-miRNA network comprising 1544 pairs was established.
Conclusions:
- The identified DEcircRNAs and their associated pathways provide novel insights into ischemic stroke mechanisms.
- Specific circRNAs demonstrate potential as diagnostic biomarkers for ischemic stroke.
- Further research into circRNA-miRNA interactions may reveal therapeutic targets for ischemic stroke.
