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Liquid Absorbance Standards.

R W Burke1, E R Deardorff1, O Menis1

  • 1Institute for Materials Research, National Bureau of Standards, Washington, D.C. 20234.

Journal of Research of the National Bureau of Standards. Section A, Physics and Chemistry
|September 27, 2021
PubMed
Summary
This summary is machine-generated.

This study quantifies errors in liquid filter absorbance measurements. Understanding these instrumental and chemical uncertainties is crucial for accurate spectrophotometry and developing reliable absorbance standards.

Keywords:
Absorptivity dataStandard Reference Materialsaccuracyliquid absorbance standards

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Area of Science:

  • Analytical Chemistry
  • Spectrophotometry

Background:

  • Accurate absorbance measurements are vital in spectrophotometry.
  • Instrumental and chemical factors introduce errors in liquid filter absorbance.
  • Standard Reference Materials are needed for validating spectrophotometric methods.

Purpose of the Study:

  • To systematically investigate instrumental and chemical variables affecting liquid filter absorbance.
  • To quantify the magnitude of errors from specific sources.
  • To facilitate the development of reliable liquid absorbance standards.

Main Methods:

  • Analysis of three types of liquid filters: pure compounds, mixtures, and organic dyes.
  • Utilizing NBS-calibrated glass filters to establish absorptivity data accuracy.
  • Delineating error contributions from spectral bandpass, beam geometry, stray light, internal reflections, and refractive index.

Main Results:

  • Quantified errors arising from specific instrumental and chemical uncertainties.
  • Established the accuracy of absorptivity data using calibrated standards.
  • Identified key variables impacting absorbance measurement reliability.

Conclusions:

  • A systematic understanding of error sources improves absorbance measurement accuracy.
  • The study led to the development of NBS Standard Reference Material 931.
  • NBS Standard Reference Material 931 provides reliable liquid absorbance standards for UV-Vis spectrophotometry.