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Updated: Oct 19, 2025

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Trefoil Factor 3 Inhibits Thyroid Cancer Cell Progression Related to IL-6/JAK/STAT3 Signaling Pathway

Yunchao Xin1, Xiaoling Shang1, Xiaoran Sun2

  • 1Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei 075000, China.

Evidence-Based Complementary and Alternative Medicine : Ecam
|September 27, 2021
PubMed
Abstract

Insights

Trefoil factor 3 (TFF3) inhibits thyroid cancer progression by suppressing the IL-6/JAK/STAT3 pathway. Low TFF3 expression correlates with nodal metastasis and poorer survival in thyroid cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Abnormal trefoil factor 3 (TFF3) expression is implicated in various cancers, suggesting a role in tumor development and angiogenesis.
  • The specific function of TFF3 in thyroid cancer remains largely unexplored.

Purpose of the Study:

  • To investigate the role and mechanism of TFF3 in thyroid cancer progression.
  • To determine the association between TFF3 expression and clinical outcomes in thyroid cancer patients.

Main Methods:

  • TFF3 expression analyzed using GEPIA and RT-PCR.
  • Thyroid cancer cell motility and apoptosis assessed via cell function assays.
  • Mechanism explored through GSEA pathway analysis and western blot.

Main Results:

  • Low TFF3 expression in thyroid cancer is linked to nodal metastasis and reduced disease-free survival.
  • Reduced TFF3 expression enhanced cell motility and inhibited apoptosis.
  • TFF3 suppressed IL-6, p-JAK2/JAK2, and pSTAT3/STAT3 levels in thyroid cancer cells.

Conclusions:

  • TFF3 acts as a tumor suppressor in thyroid cancer.
  • TFF3 inhibits thyroid cancer cell progression by modulating the IL-6/JAK/STAT3 signaling pathway.

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