Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Oct 19, 2025

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
08:44

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

Published on: June 5, 2018

70.1K

Rapid micro-immunohistochemistry.

Robert D Lovchik1, David Taylor1,2, Govind Kaigala1

  • 1IBM Research Europe, Saeumerstrasse 4, 8803 Rueschlikon, Switzerland.

Microsystems & Nanoengineering
|September 27, 2021
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

12.0K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
12.0K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.6K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EnGRAM: Engineering Open-Space Microfluidic Gradient Modulators.

Analytical chemistry·2025
Same author

Reconfigurable microfluidics.

Nature reviews. Chemistry·2023
Same author

Quantifying Antibody Binding Kinetics on Fixed Cells and Tissues <i>via</i> Fluorescence Lifetime Imaging.

Analytical chemistry·2022
Same author

Selective Extraction of Biomolecules Using a Bidirectional Flow Filter.

Analytical chemistry·2022
Same author

Temperature dependence of diffusiophoresis <i>via</i> a novel microfluidic approach.

Lab on a chip·2022
Same author

Microscale hydrodynamic confinements: shaping liquids across length scales as a toolbox in life sciences.

Lab on a chip·2022
Same journal

Mechanistic insights into cellular deformation enable enhanced extensional-flow cytometry for label-free classification and sorting.

Microsystems & nanoengineering·2026
Same journal

AlGaN/GaN HEMT H₂ sensor with integrated Wheatstone bridge and on-chip microheater for 0.1-ppm detection.

Microsystems & nanoengineering·2026
Same journal

Fully flexible large-area MEMS-based triaxial force sensor compatible with flat panel display manufacturing.

Microsystems & nanoengineering·2026
Same journal

Self-aligned assembly of piezoelectric nanorods for 6G wireless communications.

Microsystems & nanoengineering·2026
Same journal

Wearable ultrasound: a review of core technologies and clinical applications in cardiovascular monitoring.

Microsystems & nanoengineering·2026
Same journal

Microfluidic encapsulation of the human gut microbiota-a tool for research and beyond.

Microsystems & nanoengineering·2026
See all related articles

We developed rapid immunohistochemistry (IHC) using microfluidic probes, reducing staining times to under 30 minutes. This innovation enables faster protein detection in tissues, potentially aiding surgical decisions and multiplexed assays.

Area of Science:

  • Biomedical Engineering
  • Histopathology
  • Biochemistry

Background:

  • Immunohistochemistry (IHC) is crucial for detecting specific proteins in tissue sections.
  • Standard IHC protocols involve lengthy incubation steps, limiting rapid analysis.
  • There is a need for faster, more efficient IHC methods, especially for intraoperative applications.

Purpose of the Study:

  • To develop and validate a rapid and localized immunohistochemical staining method.
  • To significantly reduce the incubation times of key IHC steps.
  • To explore the potential of this method for intraoperative analysis and multiplexed assays.

Main Methods:

  • Fabrication of horizontally oriented microfluidic probes (MFPs) with designed apertures.
  • Application of MFPs for localized and time-optimized exposure of tissue sections to biochemicals.
Keywords:
ChemistryElectrical and electronic engineering

More Related Videos

Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals &#8722; Using Unconjugated Primary Antibodies from the Same Host Species
07:37

Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species

Published on: February 21, 2020

7.1K
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

17.4K

Related Experiment Videos

Last Updated: Oct 19, 2025

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
08:44

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

Published on: June 5, 2018

70.1K
Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals &#8722; Using Unconjugated Primary Antibodies from the Same Host Species
07:37

Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species

Published on: February 21, 2020

7.1K
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

17.4K
  • Validation of the rapid IHC protocol on MDAMB468-1510A cell block sections.

    • Main Results:

    • Achieved reduction of two main IHC incubation steps to less than 30 minutes.
    • Demonstrated successful protein detection with rapid incubation times.
    • Showcased conservative tissue usage suitable for multiplexed assays.

    Conclusions:

    • The developed rapid IHC method offers a versatile and efficient alternative to standard protocols.
    • This technique has the potential for real-time application during surgery for dynamic clinical decision-making.
    • The method supports multiplexed assays, optimizing conditions with minimal tissue samples.