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Updated: Oct 19, 2025
Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
Published on: November 29, 2012
Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs
Asaf Grupi1,2, Zehavit Shapira1,2, Shimon Yudovich1,2
1Department of Physics, Institute for Nanotechnology and Advanced Materials.
Abstract:
Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABAA antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.
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