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miR-3929 Inhibits Proliferation and Promotes Apoptosis by Downregulating Cripto-1 Expression in Cervical Cancer Cells

Ying Wang1, Xiaoli Li1, Shuyue Wang2

  • 1National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, China.

Cytogenetic and Genome Research
|September 27, 2021
PubMed

Insights

MicroRNA-3929 (miR-3929) inhibits cervical cancer progression by targeting Cripto-1. This microRNA reduces tumor growth, proliferation, and induces apoptosis, offering a potential therapeutic strategy for cervical cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Cripto-1 is overexpressed in various cancers, making its downregulation a potential cancer treatment strategy.
  • The post-transcriptional regulation of Cripto-1 expression, particularly by microRNAs, is not well understood.

Purpose of the Study:

  • To investigate the post-transcriptional regulation of Cripto-1 expression by microRNAs.
  • To analyze the role of miR-3929 in regulating Cripto-1 expression and its impact on cervical cancer cell behavior.

Main Methods:

  • Bioinformatic analysis to predict miRNAs targeting Cripto-1 mRNA 3'UTR.
  • In vitro studies using cervical cancer cells to assess the effects of miR-3929 and Cripto-1.
  • In vivo studies using HeLa xenograft models in nude mice.

Main Results:

  • miR-3929 directly binds to the 3'UTR of Cripto-1 mRNA and downregulates its expression in cervical cancer cells.
  • miR-3929 inhibits cervical cancer cell viability, DNA synthesis, and Ki67 expression, inducing G2/M phase cell cycle arrest.
  • miR-3929 promotes apoptosis via the mitochondrial pathway, an effect reversed by Cripto-1 overexpression.
  • In vivo, miR-3929 significantly inhibits the growth of HeLa xenograft tumors.

Conclusions:

  • miR-3929 acts as a tumor suppressor in cervical cancer by downregulating Cripto-1.
  • Targeting Cripto-1 with miR-3929 presents a promising therapeutic avenue for cervical cancer treatment.
Keywords:
ApoptosisCervical cancerCripto-1ProliferationmiR-3929

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