Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rapidly Varying Flow01:24

Rapidly Varying Flow

173
Rapidly varying flow (RVF) in open channels is characterized by abrupt changes in flow depth over a short distance, with the rate of depth change relative to distance often approaching unity. These flows are inherently complex due to their transient and multi-dimensional nature, making exact analysis difficult. However, approximate solutions using simplified models provide valuable insights into their behavior.Key Features of Rapidly Varying FlowRVF is commonly observed in scenarios involving...
173
Free Jet01:14

Free Jet

290
Free jets describe the flow of liquid exiting a reservoir through an opening into the atmosphere without resistance. The velocity (v) of the liquid jet is derived using Bernoulli's principle and expressed as:
290

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanism of Activation and Mechanical Properties of Alkali-Activated Material Derived from GGBFS/FA Activated by Carbide Slag.

Materials (Basel, Switzerland)·2026
Same author

Extracorporeal Shock Wave Therapy Promotes Peripheral Nerve Regeneration Through ERK1/2-Mediated Autophagy.

Annals of the New York Academy of Sciences·2026
Same author

A study on the hydraulic and pollutant removal performance of bioretention systems using plants and oyster shells.

Journal of environmental management·2026
Same author

A Mechanistic Investigation on Cation-Modified Cellulose Nanofibrils-Reinforced Cement Composite.

Materials (Basel, Switzerland)·2026
Same author

Multiscale Effect of Carboxyl Modification on the Mechanical Properties of Palm Oil-Based Elastomers Studied by Molecular Dynamics Simulation.

The journal of physical chemistry. B·2025
Same author

Quality characters of low-salt semi-dried golden pomfret processed with an innovational substitute of plasma-activated water.

Food chemistry: X·2025

Related Experiment Video

Updated: Oct 19, 2025

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
09:33

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

Published on: September 11, 2020

6.4K

DNA fragmentation in complicated flow fields created by micro-funnel shapes.

Shuyi Wu1, Tengfei Fu1, Renhui Qiu1

  • 1College of Transportation and Civil Engineering, Fujian Agriculture and Forestry University, Fuzhou, 350108, China.

Soft Matter
|September 27, 2021
PubMed
Summary

Micro-funnel shape significantly impacts DNA fragmentation by altering flow fields. Understanding these dynamics aids in designing better microfluidic devices for DNA manipulation and gene sequencing.

More Related Videos

Fragmenting Bulk Hydrogels and Processing into Granular Hydrogels for Biomedical Applications
10:18

Fragmenting Bulk Hydrogels and Processing into Granular Hydrogels for Biomedical Applications

Published on: May 17, 2022

6.1K
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.3K

Related Experiment Videos

Last Updated: Oct 19, 2025

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
09:33

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

Published on: September 11, 2020

6.4K
Fragmenting Bulk Hydrogels and Processing into Granular Hydrogels for Biomedical Applications
10:18

Fragmenting Bulk Hydrogels and Processing into Granular Hydrogels for Biomedical Applications

Published on: May 17, 2022

6.1K
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

11.3K

Area of Science:

  • Biophysics
  • Microfluidics
  • Molecular Biology

Background:

  • Micro-funnels are crucial for DNA manipulation, enabling processes like DNA mapping and gene sequencing.
  • A systematic understanding of DNA fragmentation in complex microfluidic flow fields is lacking, hindering device design.

Purpose of the Study:

  • To investigate the effect of different micro-funnel shapes on DNA fragmentation.
  • To elucidate the relationship between flow field characteristics and DNA fragmentation dynamics.

Main Methods:

  • Experiments using lambda DNA fragmentation in microfluidic chips with four distinct funnel shapes.
  • Numerical simulations employing Brownian dynamics-computational fluid dynamics to analyze flow fields.

Main Results:

  • Micro-funnel shape significantly influences DNA fragment size.
  • Flow field extension rate magnitude and distribution are altered by funnel geometry.
  • Micro-scale extension rate is the dominant factor in DNA fragmentation.

Conclusions:

  • Funnel shape critically impacts DNA fragmentation behavior, including fragment size, location, and molecular conformation.
  • This research provides insights for the rational design of microfluidic devices for DNA manipulation.