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Antifouling Aptasensor Based on Self-Assembled Loop-Closed Peptides with Enhanced Stability for CA125 Assay in
Min Chen1, Rui Han1, Wenqi Wang1
1Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE; Shandong Key Laboratory of Biochemical Analysis; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.
Analytical Chemistry
|September 27, 2021
Summary
Researchers developed an ultralow fouling biosensor using self-assembling peptides. This stable, loop-structured peptide platform enhances detection in complex biological fluids like serum.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Biosensing
Background:
- Biosensors often suffer from fouling in complex biological fluids, limiting their practical application.
- Antifouling strategies are crucial for developing robust and reliable biosensing platforms.
- Peptide self-assembly offers a versatile approach for surface modification and biosensor design.
Purpose of the Study:
- To develop a universal, ultralow fouling sensing platform for analyzing targets in complex biofluids.
- To create a stable, self-assembled peptide structure with enhanced antifouling properties.
- To construct a sensitive electrochemical aptasensor for cancer antigen 125 (CA125) detection.
Main Methods:
- Designed antifouling peptides with terminal thiol groups for self-assembly.
- Utilized gold nanoparticle (AuNP)-modified electrodes and electrodeposited poly(3,4-ethylenedioxythiophene) (PEDOT).
- Co-immobilized peptides and aptamers onto the modified electrode surface for aptasensor construction.
Main Results:
- Achieved excellent antifouling performance and stability in clinical human serum.
- Developed a sensitive electrochemical aptasensor for CA125 detection.
- Demonstrated a wide linear range (0.1-1000 U mL⁻¹) and low limit of detection (0.027 U mL⁻¹).
Conclusions:
- The loop-closed structure of self-assembled peptides provides effective antifouling capabilities.
- The developed peptide-based aptasensor shows potential for accurate CA125 detection in clinical samples.
- This antifouling strategy paves the way for advanced biosensors in complex biological fluid analysis.
