Related Experiment Video
Updated: Oct 18, 2025

Electrochemically and Bioelectrochemically Induced Ammonium Recovery
Published on: January 22, 2015
Microfluidic aqueous two-phase system-based nitrifying bacteria encapsulated colloidosomes for green and sustainable
Gang Liu1, Ting Du1, Jialin Chen1
1School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031, PR China.
Abstract:
A novel strategy was proposed for preparing micro-scale monodisperses nitrifying bacteria (NB) encapsulated Ca-Alg@CaCO3 colloidosomes by exploiting capillary microfluidic device, as an attempt to treat ammonium-nitrogen wastewater in an environment-friendly, efficient and repeatable manner based on the aqueous two-phase (ATPS) system. By complying with the spatial confined urease mediate biomineralization reactions, ATPS droplets (Dextran in Polyethylene glycol) containing urease, NB regent and alginate were used as templates to prepare 500 μm Ca-Alg@CaCO3 colloidosomes with 16.48 Mpa mechanical strength. The activity of NB encapsulated in the colloidosomes was high. The simulated wastewater treated with the colloidosomes achieved a high removal rate even at harsh temperature and pH value. In both simulated and real wastewater treatment, prolonged reuse times (216 h) with high removal rate (>90%, after being applied 72 h) were obtained by using Ca-Alg@CaCO3 colloidosomes, as compared with that (96 h) by using general alginate microbeads.
More Related Videos
08:13A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
08:05Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
Related Concept Videos
Metabolism of Chemolithotrophs
Environmental Applications of Microorganisms
Inorganic Nitrogen Assimilation
Coagulation