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Updated: Oct 18, 2025
Scalable Step-by-Step Approach of Sustainable Bioplastic Production from Food Waste
Published on: July 18, 2025
Exploring the feasibility of nitrous oxide reduction and polyhydroxyalkanoates production simultaneously by mixed
Fang Fang1, Run-Ze Xu1, Yan-Qiu Huang2
1Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
Abstract:
Nitrous oxide (N2O), as a powerful greenhouse gas, has drawn increasing attention in recent years and different strategies for N2O reduction were explored. In this study, a novel strategy for valuable polyhydroxyalkanoates (PHA) production coupling with N2O reduction by mixed microbial cultures (MMC) using different substrates was evaluated. Results revealed that N2O was an effective electron acceptor for PHA production. The highest PHA yield (0.35 Cmmol PHA/Cmmol S) and PHA synthesis rate (227.47 mg PHA/L/h) were obtained with acetic acid as substrate. Low temperature (15℃) and pH of 8.0 were beneficial for PHA accumulation. Results of the thermogravimetric analysis showed that PHA produced with N2O as electron acceptor has better thermal stability (melting temperature of 99.4℃ and loss 5% weight temperature of 211.4℃). Our work opens up new avenues for simultaneously N2O reduction and valuable bioplastic production, which is conducive to resource recovery and climate protection.
