Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

15.3K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
15.3K
Mitochondria01:37

Mitochondria

15.8K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
15.8K
Mitochondrial Membranes01:45

Mitochondrial Membranes

13.1K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
13.1K
Hypoxia01:23

Hypoxia

1.4K
Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CitNAC71-CitNAC76 coordinate cellulose and hemicellulose biosynthesis to regulate high-temperature-mediated granulation in citrus.

The Plant cell·2026
Same author

Mitochondrial dysfunction in Alzheimer's disease: targeting the powerhouse with nanomedicine.

Frontiers in pharmacology·2026
Same author

New Insights into the Mechanisms of Bitterness and Astringency in Chitosan Oligosaccharide Complexes.

Journal of agricultural and food chemistry·2026
Same author

Spatial organization of NLR signaling complexes in plant immunity.

Journal of integrative plant biology·2026
Same author

Integrated Characterization and Pharmacokinetics of Kanli Granule in Rats Based on UPLC-Q-TOF-MS/MS: In Vitro and In Vivo Component Screening.

Biomedical chromatography : BMC·2026
Same author

Pharmacokinetics and homeostatic impact of golden bile powder: evidence from surrogate analyte-based UPLC-MS/MS in rats.

Chinese medicine·2026
Same journal

GPC3 chimeric antigen receptor (CAR)-NK cells combined with Enoblituzumab enhance the anti-tumor efficacy against hepatocellular carcinoma.

Biochemical and biophysical research communications·2026
Same journal

A miR-382-5p-PORCN axis modulates endogenous Wnt signaling during palatal development.

Biochemical and biophysical research communications·2026
Same journal

Development of a vitamin-related gene signature to predict the immune characteristics and prognosis of glioma.

Biochemical and biophysical research communications·2026
Same journal

Cyclo(Ser-Tyr) L/D spatial isomerism exerts opposing effects on benign prostatic hyperplasia.

Biochemical and biophysical research communications·2026
Same journal

Necrotic tumor cells contribute to pro-tumoral responses and inflammatory mediator production via NF-κB activation in oral squamous cell carcinoma.

Biochemical and biophysical research communications·2026
Same journal

GPR4 promotes ferroptosis in nucleus pulposus cells via MAPK-dependent mitophagy suppression.

Biochemical and biophysical research communications·2026
See all related articles

Related Experiment Video

Updated: Oct 18, 2025

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
05:59

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

3.1K

TASK-1 regulates mitochondrial function under hypoxia.

Yang Yu1, Harrison He1, Chen Kang1

  • 1Division of Pharmaceutics and Pharmacology, College of Pharmacy, The Ohio State University, Columbus, OH, 43210, USA.

Biochemical and Biophysical Research Communications
|September 27, 2021
PubMed
Summary
This summary is machine-generated.

TWIK-related acid-sensitive potassium channel 1 (TASK-1) protects heart cells from hypoxia-reoxygenation injury. TASK-1 maintains mitochondrial function and viability by inhibiting the mitochondrial permeability transition pore.

Keywords:
Cytochrome cHypoxiaMitochondriaMitochondrial membrane potentialTASK-1

More Related Videos

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
07:14

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Published on: July 13, 2018

14.6K
Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics
07:03

Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics

Published on: August 23, 2024

1.1K

Related Experiment Videos

Last Updated: Oct 18, 2025

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
05:59

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

3.1K
A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
07:14

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Published on: July 13, 2018

14.6K
Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics
07:03

Author Spotlight: Uncovering the Role of Mitochondrial Calcium Phosphate in Heart Failure and Bioenergetics

Published on: August 23, 2024

1.1K

Area of Science:

  • Cardiovascular Science
  • Cell Biology
  • Molecular Physiology

Background:

  • TWIK-related acid-sensitive potassium channel 1 (TASK-1) is a constitutively active two-pore-domain potassium channel.
  • TASK-1 is highly expressed in the heart, but its role in hypoxia remains unclear.

Purpose of the Study:

  • To investigate the role of TASK-1 channels in cytoprotection against hypoxia-reoxygenation (H/R) injury in cardiac cells.
  • To elucidate the underlying mechanisms of TASK-1-mediated protection, focusing on mitochondrial function.

Main Methods:

  • Utilized a cellular model of H/R using rat heart-derived H9c2 cells and TASK-1 deficient HEK293T cells.
  • Assessed cell viability using cell viability assays.
  • Evaluated mitochondrial membrane potential (MMP) via tetramethylrhodamine fluorescence.
  • Determined cytochrome c release and mitochondrial-pharmacological interventions.

Main Results:

  • TASK-1 expression significantly increased cell viability under H/R conditions.
  • TASK-1 expression maintained MMP during H/R, similar to cyclosporin A, an mPTP inhibitor.
  • TASK-1 expression inhibited H/R-induced cytochrome c release.
  • TASK-1 showed partial colocalization with mitochondria, unaffected by H/R.

Conclusions:

  • TASK-1 channels confer significant cytoprotection against hypoxia-reoxygenation injury in cardiac cells.
  • TASK-1 likely protects mitochondria by inhibiting the mitochondrial permeability transition pore (mPTP) opening, thereby maintaining MMP.
  • These findings highlight TASK-1 as a potential therapeutic target for cardiac ischemia-reperfusion injury.