Related Experiment Video
Updated: May 5, 2026
06:16
Preparation Of Gushukang GSK Granules for In Vivo and In Vitro Experiments
Published on: May 9, 2019
6.8K
Integrated Characterization and Pharmacokinetics of Kanli Granule in Rats Based on UPLC-Q-TOF-MS/MS: In Vitro and
Xuehao Cheng1, Kexin Xia1,2, Xiaofen Ruan3
1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Biomedical Chromatography : BMC
|May 4, 2026
Summary
This study identified key compounds in Kanli granules (KLG) for chronic heart failure treatment. It details KLG
Area of Science:
- Pharmacology and Phytochemistry
- Traditional Chinese Medicine Research
Background:
- Kanli granules (KLG) is a widely used traditional Chinese medicine preparation for treating chronic heart failure (CHF).
- Understanding the material basis and active components of KLG is crucial for its rational clinical application and further development.
Purpose of the Study:
- To elucidate the comprehensive chemical composition of KLG.
- To identify the key bioactive components responsible for KLG's therapeutic effects in chronic heart failure.
- To investigate the pharmacokinetics of major KLG constituents.
Main Methods:
- Ultra-Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-TOF-MS/MS) for chemical profiling.
- Network pharmacology and literature mining to screen key active components.
- Quantitative UPLC-MS/MS method development and pharmacokinetic studies in rats.
Main Results:
- Identified 288 compounds in KLG, with 43 detected in rat plasma, 99 in urine, and 59 in bile.
- Screened nine key components, including paeoniflorin and atractylenolide III, as potentially responsible for therapeutic effects.
- Pharmacokinetic analysis revealed rapid absorption for six tested compounds, with significant gender-related differences in metabolism for hypaconine and mangiferin.
Conclusions:
- This study provides the first comprehensive chemical composition spectrum of KLG.
- Identified potential bioactive compounds and elucidated the material basis of KLG for chronic heart failure.
- Offers new insights for the research of material basis and active component screening of Chinese herbal compound preparations.
