Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oxygen Transport in the Blood01:27

Oxygen Transport in the Blood

4.2K
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
4.2K
Oxygen Delivering System II: Venturi Mask and Transtracheal Oxygen01:16

Oxygen Delivering System II: Venturi Mask and Transtracheal Oxygen

1.1K
Oxygen therapy is a pivotal aspect of medical care, particularly for patients with respiratory ailments. Two prominent oxygen-delivering systems include the Venturi mask and the transtracheal oxygen catheter.
Venturi Mask
The Venturi mask, named after the Venturi effect, is designed to deliver precise oxygen concentrations. It consists of a large tube with an oxygen inlet that narrows down, causing a pressure drop that pulls air in through adjustable side ports. The mask is a lightweight,...
1.1K
Oxygen Delivering System I: Nasal Cannula and Face Mask01:26

Oxygen Delivering System I: Nasal Cannula and Face Mask

751
The human body requires oxygen to function, and when the natural process of respiration is hindered, external devices, including the following, are needed to help deliver this vital gas.
Nasal Cannula
A nasal cannula is a lightweight tube split at one end into two prongs and placed in the nostrils. It is typically used to deliver low to medium levels of oxygen.
Suggested flow rate: The suggested flow rate for a nasal cannula typically ranges between 1 and 6 L/min.
Oxygen percentage setting:...
751
Gas Exchange and Transport01:20

Gas Exchange and Transport

72.8K
Gas exchange, the intake of molecular oxygen (O2) from the environment and the outflow of carbon dioxide (CO2) into the environment, is necessary for cellular function. Gas exchange during respiration occurs largely via the movement of gas molecules along pressure gradients. Gas travels from areas of higher partial pressure to areas of lower partial pressure. In mammals, gas exchange occurs in the alveoli of the lungs, which are adjacent to capillaries and share a membrane with them.
72.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Prisciandaro et al. Anatomical Versus Non-Anatomical Pulmonary Metastasectomy: European Multicentre Analysis. <i>Cancers</i> 2026, <i>18</i>, 1037.

Cancers·2026
Same author

Metastatic Trajectories in Non-Small Cell Lung Cancer Guide Local and Systemic Therapies.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2026
Same author

Multiplexed single-cell and spatial profiling reveal B cells and tertiary lymphoid structures as prognostic indicators in pleural mesothelioma.

British journal of cancer·2026
Same author

Anatomical Versus Non-Anatomical Pulmonary Metastasectomy: European Multicentre Analysis.

Cancers·2026
Same author

Refining Risk Stratification and Surveillance Strategies in Pleuropulmonary Solitary Fibrous Tumors-An International, Retrospective, Multicenter Analysis.

Cancers·2025
Same author

Pleural mesothelioma.

Nature reviews. Disease primers·2025
Same journal

RETRACTED: Bakshi et al. Crocin Inhibits Angiogenesis and Metastasis in Colon Cancer via TNF-α/NF-kB/VEGF Pathways. <i>Cells</i> 2022, <i>11</i>, 1502.

Cells·2026
Same journal

Correction: Verde et al. Molecular Mechanisms of Protein Aggregation in ALS-FTD: Focus on TDP-43 and Cellular Protective Responses. <i>Cells</i> 2025, <i>14</i>, 680.

Cells·2026
Same journal

Inflammation in Cardiomyopathies: Cellular Mechanisms Across Cardiac Phenotype.

Cells·2026
Same journal

IL-4/IL-13-Driven Dysregulation of Epidermal Lipid Metabolism in Atopic Dermatitis: An Immunometabolic Link Between Type 2 Inflammation and Barrier Dysfunction.

Cells·2026
Same journal

Activity of DNA- and RNA-Guided Prokaryotic Argonautes in Human Mitochondria.

Cells·2026
Same journal

Placental Pathophysiology in Maternal Psychoactive Substance Use: Biological, Clinical, and Forensic Perspectives.

Cells·2026
See all related articles

Related Experiment Video

Updated: Oct 18, 2025

Normothermic Negative Pressure Ventilation Ex Situ Lung Perfusion: Evaluation of Lung Function and Metabolism
09:31

Normothermic Negative Pressure Ventilation Ex Situ Lung Perfusion: Evaluation of Lung Function and Metabolism

Published on: February 14, 2022

2.2K

Perfluorocarbon-Based Oxygen Carriers and Subnormothermic Lung Machine Perfusion Decrease Production of

Stephan Arni1, Citak Necati1, Tatsuo Maeyashiki1

  • 1Department of Thoracic Surgery, University Hospital Zürich, 8091 Zürich, Switzerland.

Cells
|September 28, 2021
PubMed
Summary
This summary is machine-generated.

Subnormothermic ex vivo lung perfusion (EVLP) using perfluorocarbon-based oxygen carriers (PFCOC) at 28°C improves lung donor quality. This method enhances lung physiology and reduces inflammation compared to normothermic EVLP.

Keywords:
ex vivo lung perfusionlung transplantationperfluorocarbon-based oxygen carrierssubnormothermic perfusion

More Related Videos

Author Spotlight: Utilizing Next-Generation Polymerized Human Hemoglobin for Improved Donor Lung Evaluation and Preservation in Rats
09:47

Author Spotlight: Utilizing Next-Generation Polymerized Human Hemoglobin for Improved Donor Lung Evaluation and Preservation in Rats

Published on: June 14, 2024

764
Ex Situ Normothermic Machine Perfusion of Donor Livers
12:13

Ex Situ Normothermic Machine Perfusion of Donor Livers

Published on: May 26, 2015

11.9K

Related Experiment Videos

Last Updated: Oct 18, 2025

Normothermic Negative Pressure Ventilation Ex Situ Lung Perfusion: Evaluation of Lung Function and Metabolism
09:31

Normothermic Negative Pressure Ventilation Ex Situ Lung Perfusion: Evaluation of Lung Function and Metabolism

Published on: February 14, 2022

2.2K
Author Spotlight: Utilizing Next-Generation Polymerized Human Hemoglobin for Improved Donor Lung Evaluation and Preservation in Rats
09:47

Author Spotlight: Utilizing Next-Generation Polymerized Human Hemoglobin for Improved Donor Lung Evaluation and Preservation in Rats

Published on: June 14, 2024

764
Ex Situ Normothermic Machine Perfusion of Donor Livers
12:13

Ex Situ Normothermic Machine Perfusion of Donor Livers

Published on: May 26, 2015

11.9K

Area of Science:

  • Transplantation immunology
  • Organ preservation
  • Biomedical engineering

Background:

  • Clinical assessment of marginal donor lungs relies on normothermic machine perfusion.
  • Subnormothermic temperatures and perfluorocarbon-based oxygen carriers (PFCOC) show promise in other organ transplants.
  • Their efficacy in ex vivo lung perfusion (EVLP) needs further study.

Purpose of the Study:

  • To evaluate the effects of PFCOC with 28°C or 37°C perfusion during EVLP in a rat model.
  • To determine if subnormothermic EVLP with PFCOC improves lung donor physiology and tissue parameters.

Main Methods:

  • A 4-hour EVLP in a rat model using perfluorocarbon-based oxygen carriers (PFCOC).
  • Comparison of perfusion at 28°C versus 37°C.
  • Assessment of pulmonary vascular resistance (PVR), dynamic compliance (Cdyn), biochemical markers (potassium, lactate, ATP), and inflammatory markers (myeloperoxidase, TNFα, IL-6, IL-7, etc.).

Main Results:

  • Subnormothermic EVLP (28°C) with PFCOC showed significantly lower PVR, higher Cdyn, lower potassium and lactate, higher ATP, and lower myeloperoxidase activity compared to 37°C EVLP with PFCOC.
  • Subnormothermic EVLP (with or without PFCOC) significantly reduced pro-inflammatory mediators (TNFα, IL-6, IL-7) and chemokines.
  • 28°C EVLP improved Cdyn and PVR, decreased pro-inflammatory cytokines and pCO2, and reduced myeloperoxidase activity.

Conclusions:

  • Subnormothermic EVLP using PFCOC at 28°C significantly enhances lung donor physiology.
  • This approach ameliorates biochemical and inflammatory parameters in lung tissue.
  • Subnormothermic EVLP with PFCOC represents a promising strategy for improving donor lung quality.