Related Experiment Video

Updated: Oct 18, 2025

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

27.8K

HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis

Aida Barreiro-Alonso1,2,3,4, Mónica Lamas-Maceiras1,2,3, Lidia Lorenzo-Catoira1,2,3

  • 1EXPRELA Group, Centro de Investigaciones Científicas Avanzadas (CICA), University of A Coruña (UDC), 15008 A Coruña, Spain.

Cancers
|September 28, 2021
PubMed

Insights

High-mobility group box 1 (HMGB1) interacts with proteins involved in chromatin stability, transcription, and RNA processing in cancer cells. These HMGB1 interactions are dysregulated in ovarian and prostate tumors, impacting cancer progression.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Proteomics

Background:

  • High-mobility group box 1 (HMGB1) is a nuclear protein with established roles in chromatin stability and transcription.
  • The full spectrum of HMGB1 interactions and their functional implications in cancer remain incompletely understood.

Purpose of the Study:

  • To identify and characterize the HMGB1 interactome in prostate and ovary cancer cell lines.
  • To investigate the functional significance of HMGB1 interactions in cancer progression.

Main Methods:

  • Affinity purification coupled with mass spectrometry (AP-MS) to identify HMGB1 interacting proteins.
  • Yeast-two-hybrid assays to validate specific protein-protein interactions.
  • Analysis of Pan-Cancer Atlas data to correlate gene expression with clinical outcomes.

Main Results:

  • The HMGB1 nuclear interactome includes proteins involved in chromatin stability, transcription, and novel roles in mRNA and rRNA processing.
  • Identified interactions between HMGB1 and the NuRD complex (specifically RBBP7), and between the septin and THOC complexes with Rab11.
  • Dysregulation of genes encoding HMGB1-interacting proteins in ovarian and prostate carcinomas was observed.
  • Silencing HMGB1 in PC-3 cells downregulated key regulators of ribosome biogenesis and RNA processing (BOP1, RSS1, UBF1, KRR1, LYAR).

Conclusions:

  • HMGB1 plays a significant role in RNA processing, beyond its known functions in chromatin and transcription.
  • Novel HMGB1 interaction networks involving the NuRD, septin, THOC, and Rab11 complexes were elucidated.
  • The identified HMGB1-interacting proteins and their dysregulation in cancer highlight potential therapeutic targets and prognostic markers for prostate and ovarian carcinomas.
Keywords:
HMGB1 interactomeRNA processingovary cancerprostate cancerribosome biogenesis

More Related Videos

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells11:11

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

8.4K
miRNA Expression Analyses in Prostate Cancer Clinical Tissues11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

11.0K

Related Concept Videos

The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
9.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.3K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
23.8K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.0K