Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparing the Impact of Mouthwashes on Patients With Fixed Orthodontic Appliances: A Network Meta-Analysis.

International journal of dental hygiene·2026
Same author

Structure-guided development of an electrochemical aptasensor for Salmonella Typhi HlyE antigen detection using in silico and experimental approaches.

Scientific reports·2026
Same author

CRISPR-Cas13a-Powered Electrochemical Biosensor for Label-Free Detection of Chikungunya Virus RNA Using a Ceria-Modified Screen-Printed Carbon Electrode.

ACS sensors·2026
Same author

Assessing the potential of <i>DZIP1L</i> gene in autosomal recessive polycystic kidney disease gene therapy.

Pediatric discovery..·2025
Same author

Performance of Antibody-Detection Tests for Human Melioidosis: A Systematic Review and Meta-analysis.

The Malaysian journal of medical sciences : MJMS·2025
Same author

Isolation and characterization of DNA aptamers against the HlyE antigen of Salmonella Typhi.

Scientific reports·2024
Same journal

Correction: Luca et al. Global and Regional Diagnostic Results of Progress Toward Cervical Cancer Elimination, According to the WHO Strategy: A Systematic Literature Review with Narrative Synthesis. <i>Diagnostics</i> 2026, <i>16</i>, 1224.

Diagnostics (Basel, Switzerland)·2026
Same journal

Association Between Systemic Inflammatory Response Biomarkers and Disease Activity in Systemic Lupus Erythematosus: A Multi-Center Retrospective Study.

Diagnostics (Basel, Switzerland)·2026
Same journal

Vertebrogenic Low Back Pain and Basivertebral Nerve Ablation: A Review of Mechanisms, Imaging-Driven Selection, and Clinical Outcomes.

Diagnostics (Basel, Switzerland)·2026
Same journal

Multivalvular Carcinoid Heart Disease: The Role of Echocardiography in Diagnosis and Selection for Heterotopic Bicaval Valve Implantation.

Diagnostics (Basel, Switzerland)·2026
Same journal

Data-Efficient and Explainable Multimodal Survival Prediction in NSCLC Using Deep Image Embeddings, Clinical Variables, and Gradient-Boosted Trees.

Diagnostics (Basel, Switzerland)·2026
Same journal

Anomalous Left Coronary Artery from the Pulmonary Artery: Cinematic Volume Rendering Technique for Enhanced Anatomic Visualization.

Diagnostics (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Oct 18, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.6K

RT-LAMP CRISPR-Cas12/13-Based SARS-CoV-2 Detection Methods.

Kasturi Selvam1, Mohamad Ahmad Najib1, Muhammad Fazli Khalid1

  • 1Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.

Diagnostics (Basel, Switzerland)
|September 28, 2021
PubMed
Summary
This summary is machine-generated.

This review summarizes clustered regularly interspaced short palindromic repeats (CRISPR) and reverse-transcription loop-mediated isothermal amplification (RT-LAMP) diagnostic techniques for detecting SARS-CoV-2, offering a promising alternative to traditional COVID-19 testing.

Keywords:
COVID-19CRISPRCas12Cas13RT-LAMPSARS-CoV-2

More Related Videos

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

999
Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

958

Related Experiment Videos

Last Updated: Oct 18, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.6K
DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

999
Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
05:34

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

958

Area of Science:

  • Molecular Biology
  • Infectious Diseases
  • Biotechnology

Background:

  • Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, necessitates accurate and rapid diagnostics.
  • Current gold standard, RT-qPCR, requires specialized labs and personnel, limiting accessibility.
  • Clustered regularly interspaced short palindromic repeats (CRISPR) technology offers potential for sensitive, specific, and rapid molecular diagnostics.

Purpose of the Study:

  • To review and summarize the application of CRISPR-based diagnostic techniques for SARS-CoV-2 detection.
  • To highlight the integration of CRISPR with reverse-transcription loop-mediated isothermal amplification (RT-LAMP).
  • To assess the potential of these combined methods as rapid and accessible COVID-19 diagnostic tools.

Main Methods:

  • Literature review of CRISPR-based diagnostic techniques for SARS-CoV-2.
  • Focus on methods combining CRISPR with reverse-transcription loop-mediated isothermal amplification (RT-LAMP).
  • Analysis of sensitivity, specificity, and operational requirements of reviewed techniques.

Main Results:

  • CRISPR-based diagnostics, particularly when combined with RT-LAMP, show significant promise for SARS-CoV-2 detection.
  • These methods offer rapid results without complex laboratory infrastructure.
  • High sensitivity and specificity comparable to traditional methods are achievable.

Conclusions:

  • RT-LAMP CRISPR-based diagnostics represent a viable and advantageous alternative for widespread COVID-19 testing.
  • These technologies can improve global diagnostic capacity, especially in resource-limited settings.
  • Further development and validation are crucial for clinical implementation.