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Liquid Biopsy, ctDNA Diagnosis through NGS
Chen Lin1, Xuzhu Liu1, Bingyi Zheng2,3
1School of Medicine, Huaqiao University, Quanzhou 362021, China.
Life (Basel, Switzerland)
|September 28, 2021
Summary
Liquid biopsy using circulating tumor DNA (ctDNA) profiling with next-generation sequencing (NGS) offers a less invasive and more convenient way to analyze cancer genetics. This technology provides real-time tumor information, advancing precision medicine for cancer patients.
Area of Science:
- Oncology
- Genomics
- Molecular Diagnostics
Background:
- Liquid biopsy offers a minimally invasive alternative to traditional tissue biopsy for cancer diagnosis.
- Circulating tumor DNA (ctDNA) reflects real-time genomic alterations within a tumor.
- Next-generation sequencing (NGS) technologies are now cost-effective and performant for clinical applications.
Purpose of the Study:
- To highlight the potential of ctDNA profiling via NGS in revolutionizing clinical oncology.
- To emphasize the advantages of liquid biopsy over tissue biopsy for cancer management.
- To discuss the growing role of ctDNA analysis in precision medicine.
Main Methods:
- Profiling of circulating tumor DNA (ctDNA) using next-generation sequencing (NGS).
- Comparison of liquid biopsy techniques with traditional tissue biopsy methods.
- Assessment of ctDNA's utility across the cancer diagnosis and management continuum.
Main Results:
- ctDNA profiling by NGS provides real-time tumor genomic information.
- Liquid biopsy offers advantages including less invasiveness, ease of sampling, and reduced bias.
- NGS-based ctDNA testing is becoming increasingly suitable and popular for clinical use.
Conclusions:
- Profiling ctDNA with NGS is a biologically sound and technically convenient approach for cancer diagnosis.
- Advancements in liquid biopsy ctDNA testing will significantly benefit cancer patients.
- This technology paves the way for enhanced precision medicine strategies in oncology.
