Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in glucose transport activity of Friend erythroleukemia cell caused by dimethylsulfoxide, a

M Kasahara, P C Hinkle, Y Ikawa

    Biochimica Et Biophysica Acta
    |April 25, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Efficiency of leukocyte depletion filters and micro-aggregate filters following intra-operative cell salvage during cesarean delivery.

    International journal of obstetric anesthesia·2019
    Same author

    Mild to Moderate Intrapulmonary Shunting in Pediatric Liver Transplantation: Is Screening Necessary?

    Transplantation proceedings·2018
    Same author

    Six National University Consortium in Liver Transplant Professionals Training (SNUC-LT) Program in Japan.

    Transplantation proceedings·2018
    Same author

    Liver Transplantation for Mitochondrial Respiratory Chain Disorder: A Single-Center Experience and Excellent Marker of Differential Diagnosis.

    Transplantation proceedings·2017
    Same author

    Total internal biliary diversion during liver transplantation for type 1 progressive familial intrahepatic cholestasis: a novel approach.

    Pediatric transplantation·2016
    Same author

    [Kouichi Terazawa (1952-2015)].

    [Hokkaido igaku zasshi] The Hokkaido journal of medical science·2016

    Friend erythroleukemia cells utilize a facilitated diffusion system for D-glucose transport. Differentiation induction via dimethyl sulfoxide (Me2SO) significantly reduces glucose transport activity, suggesting a link between differentiation and transporter function.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Cancer Research

    Background:

    • Friend erythroleukemia cells are a model system for studying cellular differentiation.
    • Glucose transport is crucial for cellular metabolism and energy production.

    Purpose of the Study:

    • To characterize the D-glucose transport system in a Friend erythroleukemia cell line (T-3-C1-2-O).
    • To investigate the effect of differentiation induction on glucose transport activity.

    Main Methods:

    • Characterization of D-glucose transport kinetics, including half-saturation concentration and inhibitor sensitivity.
    • Measurement of 3-O-methyl-D-glucose transport.
    • Assessment of glucose transport activity following dimethyl sulfoxide (Me2SO) treatment.
    • Immunoblotting analysis to identify potential glucose transporter proteins.

    Related Experiment Videos

    Main Results:

    • A facilitated diffusion system for D-glucose transport was identified, with a half-saturation concentration of 2.2 mM, sensitive to mercuric ions, cytochalasin B, phloretin, and stilbestrol.
    • 3-O-methyl-D-glucose transport was more rapid than D-glucose transport.
    • Me2SO-induced differentiation in T-3-C1-2-O cells led to a significant decrease in glucose transport activity (reduced Vmax), while an induction-insensitive cell line (T-3-K-1) showed minimal changes.
    • Immunoblotting detected a 52,000 molecular weight protein band, potentially representing a glucose transporter.

    Conclusions:

    • The decrease in glucose transport activity during Me2SO-induced differentiation is associated with the differentiation process itself, not a direct effect of Me2SO.
    • Friend erythroleukemia cells express a glucose transporter that may be downregulated during differentiation.