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Surface Plasmon Resonance-Based Immunosensor for Igm Detection with Gold Nanoparticles.

Nilay Bereli1, Monireh Bakhshpour1, Aykut Arif Topçu2

  • 1Chemistry Department, Hacettepe University, Beytepe, 06800 Ankara, Turkey.

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Summary

A novel surface plasmon resonance (SPR) immunosensor using amine-functionalized gold nanoparticles detects immunoglobulin M (IgM) with high sensitivity and speed. This rapid and reusable sensor offers a promising tool for IgM detection in various solutions.

Keywords:
biosensorgold nanoparticlehuman immunoglobulin Mimmunosensorsurface plasmon resonance

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Accurate detection of immunoglobulin M (IgM) is crucial for diagnosing various diseases.
  • Existing detection methods can be time-consuming or require complex sample preparation.
  • Development of rapid, sensitive, and reusable immunosensors is highly desirable.

Purpose of the Study:

  • To develop and characterize a surface plasmon resonance (SPR) based immunosensor for sensitive detection of immunoglobulin M (IgM).
  • To evaluate the performance of the immunosensor in aqueous solutions and artificial plasma.
  • To assess the sensor's kinetic properties, reusability, and analytical detection limits.

Main Methods:

  • Immobilization of amine-functionalized gold nanoparticles (N-AuNPs) onto a sensing surface for SPR detection.
  • Characterization using scanning electron microscopy (SEM), contact angle measurements, and ellipsometry.
  • Kinetic studies and performance evaluation across a range of IgM concentrations (1.0–200 ng/mL).

Main Results:

  • The SPR immunosensor demonstrated high sensitivity with a limit of detection (LOD) of 0.08 ng/mL and a limit of quantification (LOQ) of 0.26 ng/mL.
  • Rapid analysis time of approximately 10 minutes per cycle, including adsorption, desorption, and regeneration.
  • Excellent reusability, with consistent performance over 6 consecutive adsorption-desorption and regeneration cycles.

Conclusions:

  • The developed N-AuNPs based SPR immunosensor provides a sensitive, rapid, and reusable platform for IgM detection.
  • The sensor's ability to function in both aqueous solutions and artificial plasma enhances its practical applicability.
  • Validation using enzyme-linked immunosorbent assay (ELISA) confirms the reliability of the proposed immunosensor.