Related Experiment Video
Updated: Oct 18, 2025
05:50
Pharmacophore Modeling for Targets with Extensive Ligand Libraries: A Case Study on SARS-CoV-2 Mpro
Published on: September 26, 2025
226
A BioID-derived proximity interactome for SARS-CoV-2 proteins
Danielle G May1, Laura Martin-Sancho2, Valesca Anschau2
1Enabling Technologies Group, Sanford Research, Sioux Falls, SD.
Biorxiv : the Preprint Server for Biology
|September 28, 2021
Summary
Researchers mapped interactions between SARS-CoV-2 proteins and human cells using BioID. This study provides a resource for understanding viral mechanisms and developing new COVID-19 antiviral therapies.
Area of Science:
- Virology
- Proteomics
- Cell Biology
Background:
- The COVID-19 pandemic, caused by SARS-CoV-2, presents significant global health and economic challenges.
- Understanding SARS-CoV-2 protein interactions within host cells is crucial for elucidating pathogenesis and developing antiviral treatments.
Approach:
- Utilized BioID proximity labeling to map the SARS-CoV-2 virus-host protein interactome.
- Employed human lung cancer-derived A549 cells engineered to express individual SARS-CoV-2 proteins fused with BioID.
Key Points:
- Functional enrichment analyses identified known and novel cellular pathways associated with SARS-CoV-2 proteins.
- A public website was created to host proteomic data, facilitating ongoing research into host-viral protein associations.
- The study generated comprehensive proteomic profiles of cells expressing viral-BioID fusion proteins.
Conclusions:
- This research offers a valuable resource for uncovering new SARS-CoV-2 biology.
- The findings can inform the development of novel antiviral therapies against SARS-CoV-2.
Related Concept Videos
02:26Protein Networks
4.2K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.2K
02:04Protein-protein Interfaces
14.0K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.0K
02:50Conjugated Proteins
21.2K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
21.2K
02:04Protein-Protein Interfaces
4.0K
4.0K
01:57Protein Complexes with Interchangeable Parts
2.7K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.7K
02:40Ligand Binding Sites
14.2K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
14.2K