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Development and characterization of matrix-derived microcarriers from decellularized tissues using electrospraying

Anna Kornmuller1, Lauren E Flynn1,2,3

  • 1School of Biomedical Engineering, Amit Chakma Engineering Building, The University of Western Ontario, London, Ontario, Canada.

Journal of Biomedical Materials Research. Part A
|September 28, 2021
PubMed
Summary

Researchers developed novel 3D microcarriers from tissue-specific extracellular matrix (ECM) for cell manufacturing. These ECM microcarriers support cell attachment and growth, showing promise for therapeutic cell production.

Keywords:
cell culture and deliverydecellularizationextracellular matrix (ECM)microcarrierstissue-specific

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Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Cell Therapy Manufacturing

Background:

  • Current microcarriers lack tissue-specific extracellular matrix (ECM) composition, limiting their role in directing cell function.
  • Therapeutic cell manufacturing requires advanced biomaterials that mimic native tissue environments.

Purpose of the Study:

  • To generate novel 3D microcarriers exclusively from decellularized ECM of multiple tissue sources.
  • To evaluate the characteristics, in vivo integration, and in vitro cell culture performance of these ECM-based microcarriers.

Main Methods:

  • Decellularized porcine dermis, myocardium, and human adipose tissue were digested to create ECM suspensions.
  • ECM suspensions were electrosprayed into liquid nitrogen to form 3D microcarriers without chemical crosslinking.
  • Microcarriers were characterized for mechanical properties, size, and composition, and tested in vitro and in vivo.

Main Results:

  • Generated stable, soft, and compliant 3D microcarriers from dermal, myocardial, and adipose ECM.
  • In vivo studies showed microcarrier integration into host tissue and infiltration of host cells within two weeks.
  • In vitro studies demonstrated successful attachment and proliferation of tissue-specific stromal cells on dermal and adipose microcarriers under dynamic culture conditions.

Conclusions:

  • Electrospraying is a versatile method for creating ECM-based microcarriers.
  • These novel microcarriers show potential as platforms for cell culture and delivery in therapeutic applications.
  • ECM-derived microcarriers can support host cell infiltration and tissue-specific cell growth.