Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatography: Introduction01:10

Chromatography: Introduction

5.3K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
5.3K
Chromatographic Resolution01:15

Chromatographic Resolution

1.2K
In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
1.2K
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

2.7K
Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
2.7K
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

2.7K
Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
2.7K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

835
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
835
Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

1.2K
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Constant-pattern design method for the separation of ternary mixtures of rare earth elements using ligand-assisted displacement chromatography.

Journal of chromatography. A·2018
Same author

Key parameters controlling the development of constant-pattern isotachic trains of two rare earth elements in ligand-assisted displacement chromatography.

Journal of chromatography. A·2018
Same author

Speedy standing wave design and simulated moving bed splitting strategies for the separation of ternary mixtures with linear isotherms.

Journal of chromatography. A·2017
Same author

Standing wave design and optimization of a simulated moving bed chromatography for separation of xylobiose and xylose under the constraints on product concentration and pressure drop.

Journal of chromatography. A·2017
Same author

Speedy standing wave design, optimization, and scaling rules of simulated moving bed systems with linear isotherms.

Journal of chromatography. A·2017
Same author

Design of simulated moving bed for separation of fumaric acid with a little fronting phenomenon.

Journal of chromatography. A·2017
Same journal

Impurity profiling of lipid-conjugated oligonucleotides using reversed-phase with and without ion-pair reagents and hydrophilic interaction liquid chromatography.

Journal of chromatography. A·2026
Same journal

Preparation of magnetic zwitterionic covalent organic frameworks for rapid simultaneous extraction of hydrophilic and hydrophobic organophosphates from environmental waters coupled with UHPLC-MS/MS determination.

Journal of chromatography. A·2026
Same journal

Analysis of organic and inorganic acids in biomass pyrolysis process samples by ion chromatography-mass spectrometry.

Journal of chromatography. A·2026
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026
Same journal

Ion chromatography: Current strengths, key limitations, and future trends.

Journal of chromatography. A·2026
See all related articles

Related Experiment Video

Updated: Oct 18, 2025

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.2K

Constant-pattern design method for displacement chromatography.

Yi Ding1, Nien-Hwa Linda Wang1

  • 1Davidson School of Chemical Engineering, Purdue University, 480 Stadium Mall Drive, West Lafayette, IN, 47907-2100, United States.

Journal of Chromatography. A
|September 28, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces new methods to improve displacement chromatography for chemical and biochemical purification. These advancements enable higher yields and productivity, overcoming previous limitations for commercial applications.

Keywords:
Constant-pattern design methodConstant-pattern isotachic trainMulti-zone displacement chromatography for complex mixturesSystematic splitting strategy

More Related Videos

Cell Co-culture Patterning Using Aqueous Two-phase Systems
10:11

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

18.6K
Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

16.2K

Related Experiment Videos

Last Updated: Oct 18, 2025

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
10:14

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

5.2K
Cell Co-culture Patterning Using Aqueous Two-phase Systems
10:11

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

18.6K
Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

16.2K

Area of Science:

  • Chemical Engineering
  • Separation Science

Background:

  • Displacement chromatography offers higher yields and productivity than elution chromatography.
  • Commercial applications are limited by challenges in achieving constant-pattern states and low productivity with minority components.

Purpose of the Study:

  • Develop a constant-pattern design method to overcome barriers in displacement chromatography.
  • Introduce a multi-zone displacement chromatography method to enhance productivity for mixtures with minority components.

Main Methods:

  • Theoretical analysis and dimensionless group combinations reduced parameter space.
  • Rate model simulations identified a general correlation for constant-pattern states.
  • A multi-zone approach was developed to divide separation tasks.

Main Results:

  • A predictive design method was developed, significantly increasing productivities and yields for binary mixtures.
  • The multi-zone method achieved a 680-fold increase in sorbent productivity for a ternary mixture with a minority component.

Conclusions:

  • The developed methods address key barriers to commercial displacement chromatography.
  • These advancements facilitate efficient purification of complex mixtures using displacement chromatography.