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Related Experiment Video

Updated: Oct 18, 2025

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
09:24

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Dentin-Pulp Complex Tissue Regeneration via Three-Dimensional Cell Sheet Layering.

Huijiao Yan1, Masamitsu Oshima1, Resmi Raju2

  • 1Department of Stomatognathic Function and Occlusal Reconstruction, Graduate School of Biomedical Sciences, Tokushima University, Tokushima, Japan.

Tissue Engineering. Part C, Methods
|September 29, 2021
PubMed
Summary
This summary is machine-generated.

This study engineered a layered cell sheet using dental pulp (DP) cells and odontogenic differentiation (OD) cells to regenerate the dentin-pulp complex. The layered cell sheet successfully formed organized 3D dentin-pulp-like structures, offering a promising approach for tissue engineering.

Keywords:
cell sheetdental pulp cellsdentin-pulp complexregenerationtissue engineering

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Dental Research

Background:

  • The dentin-pulp complex is vital for tooth health and can be damaged by caries and trauma, potentially leading to irreversible pulpitis and tooth loss.
  • Current treatments for damaged dentin-pulp complexes often fail to preserve pulp vitality and can compromise tooth integrity.

Purpose of the Study:

  • To fabricate a layered cell sheet combining rat dental pulp (DP) cells and odontogenic differentiation (OD) cells.
  • To evaluate the regenerative capacity of this layered cell sheet in creating a dentin-pulp complex in vivo.

Main Methods:

  • Fabrication of single DP cell sheets, single OD cell sheets, and a layered cell sheet of both cell types.
  • Characterization of cell sheets using microscopy (light, SEM) and staining (HE, IHC).
  • In vivo transplantation into immunocompromised mice, followed by evaluation of dentin-pulp regeneration using micro-CT, HE staining, and IHC after 8 weeks.

Main Results:

  • Layered cell sheets maintained cytological characteristics and demonstrated organized hard tissue formation resembling natural dentin, confirmed by micro-CT and IHC.
  • Significant increase in bone volume/tissue volume (BV/TV) in the layered cell sheet group compared to single cell sheet groups (p < 0.05).
  • Generated dentin-like tissue expressed specific dentin markers, distinct from bone markers, indicating successful regeneration.

Conclusions:

  • A bioengineered layered cell sheet composed of DP and OD cells can effectively regenerate a 3D dentin-pulp-like complex.
  • This approach shows potential for developing novel tissue engineering strategies for restoring damaged teeth.
  • The developed layered cell sheet technology offers a promising method for dentin-pulp complex regeneration, mimicking natural tooth structure.