A native mass spectrometry platform identifies HOP inhibitors that modulate the HSP90-HOP protein-protein interaction
Clinton G L Veale1, Maria Mateos-Jiménez2, Michaelone C Vaaltyn3
1FLAIR Research Fellow, School of Chemistry and Physics, Pietermaritzburg Campus, University of KwaZulu-Natal, Private Bag X01, Scottsville, 3209, South Africa. VealeC@ukzn.ac.za.
Abstract:
Herein we describe a native mass spectromery protein-peptide model as a competent surrogate for the HOP-HSP90 protein-protein interaction (PPI), application of which led to the qualititive identification of two new peptides capable of in vitro PPI disruption. This proof of concept study offers a viable alternative for PPI inhibitor screening.


