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Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
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Published on: March 31, 2021

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Differentiating Human Pluripotent Stem Cells to Vascular Endothelial Cells for Regenerative Medicine, Tissue

Taylor Bertucci1, Shravani Kakarla2, Diana Kim2

  • 1The Neural Stem Cell Institute, Rensselaer, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 30, 2021
PubMed

Vasculature plays a vital role in human biology as blood vessels transport nutrients and oxygen throughout the body. Endothelial cells (ECs), specifically, are key as they maintain barrier functions between the circulating blood and the surrounding tissues. ECs derived from human pluripotent stem cells (hPSCs) are utilized to study vascular development and disease mechanisms within in vitro models. Additionally, ECs derived from induced pluripotent stem cells (iPSCs) hold great promise for advancing personalized medicine, cell therapies, and tissue-engineered constructs by creating patient-specific cell populations. Here, we describe a xeno-free, serum-free differentiation protocol for deriving ECs from hPSCs. In brief, mesoderm progenitor cells are derived via WNT pathway activation. Following this, EC maturation is achieved with exogenous vascular endothelial growth factor A (VEGFA) and basic fibroblast growth factor 2 (bFGF2). We have characterized these cells as expressing mature EC markers and have illustrated their functionality in vitro.

Keywords:
Endothelial cellsEndothelial differentiationHuman pluripotent stem cellsWnt signalling

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