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An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers
Published on: February 26, 2018
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Quantification of In Vitro Blood-Brain Barrier Permeability
1Department of Biomedical Engineering, The City College of the City University of New York, New York, NY, USA. fu@ccny.cuny.edu.
Methods in Molecular Biology (Clifton, N.J.)
|September 30, 2021
Abstract
None:
In vitro blood-brain barrier (BBB) models have been widely used to simulate in vivo models due to their low cost, feasibility, and repeatability. To serve as a valid substitute, the in vitro BBB should have the similar barrier function as that in vivo. This chapter summarizes the detailed methods for quantifying the barrier function, e.g., the permeability of the BBB to water, ions, and solutes for an in vitro BBB generated on the Transwell filter.
Keywords:
Diffusive solute permeabilityHydraulic conductivityIn vitro blood-brain barrierTrans-BBB electric resistance (TER )Transwell filter
