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Related Experiment Videos

Developing brain produces an angiogenesis factor.

W Risau

    Proceedings of the National Academy of Sciences of the United States of America
    |June 1, 1986
    PubMed
    Summary
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    Researchers identified a novel growth factor in embryonic chicken brains that promotes capillary endothelial cell growth. This factor shows significant angiogenic capacity, crucial for blood vessel development in neural tissue.

    Area of Science:

    • Developmental Biology
    • Neuroscience
    • Angiogenesis Research

    Background:

    • Embryonic development involves complex vascularization processes.
    • Understanding growth factors is key to neural tissue development and blood-brain barrier formation.

    Purpose of the Study:

    • To identify and characterize growth factors for capillary endothelial cells in embryonic chicken brains.
    • To investigate the angiogenic potential of a novel identified growth factor.

    Main Methods:

    • Analysis of embryonic chicken brain extracts.
    • Heparin-Sepharose affinity chromatography and Sephacryl S200 gel chromatography for purification.
    • In vitro mitogenicity assays on endothelial, smooth muscle, and glial cells.
    • In vivo angiogenesis assays using rabbit cornea and chicken chorioallantoic membrane.

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    Main Results:

    • A growth factor with increasing activity from day 12 to 14-16 of embryonic development was identified.
    • High specific activity was noted early at days 3 and 4.
    • The factor, purified to 16-18 kDa, is mitogenic for endothelial cells but not smooth muscle or glial cells.
    • Demonstrated angiogenic capacity in vivo.

    Conclusions:

    • A novel endothelial cell growth factor with angiogenic properties exists in embryonic chicken brain.
    • This factor may play a role in neural tissue vascularization and blood-brain barrier development.