Dual-excitation and dual-emission carbon dots for Fe³⁺ detection, temperature sensing, and lysosome targeting

Dual-excitation and dual-emission carbon dots (CDs) have been prepared by simple one-step hydrothermal treatment of p-phenylenediamine and 5-aminosalicylic acid. The as-prepared CDs emit bright green fluorescence under excitation at 320-400 nm and bright orange fluorescence under excitation at 490-560 nm. Interestingly, the CDs can be employed as a dual-excitation and dual-emission fluorescent probe for Fe³⁺ detection in aqueous solution and living cells. Furthermore, the obtained CDs can function as a promising dual-excitation and dual-emission temperature sensor. Additionally, the CDs can be utilized for lysosomal targeting.