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A capillary driven microfluidic chip for SERS based hCG detection.

Elçin Ezgi Ahi1, Hilal Torul2, Adem Zengin3

  • 1Gebze Technical University, Faculty of Science, Department of Chemistry, 41400, Kocaeli, Turkey.

Biosensors & Bioelectronics
|September 30, 2021
PubMed
Summary
This summary is machine-generated.

A new microfluidic chip immunoassay detects Human Chorionic Gonadotropin (hCG), a WADA-prohibited substance. This method offers a sensitive and selective alternative for anti-doping analysis.

Keywords:
Capillary driven microfluidic chipHuman chorionic gonadotropin proteinMetal organic frameworkSurface enhanced Raman spectroscopy

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Materials Science

Background:

  • Human Chorionic Gonadotropin (hCG) is a prohibited substance monitored by the World Anti-Doping Agency (WADA).
  • Existing detection methods like Western Blot are time-consuming and expensive.
  • Development of rapid, sensitive, and selective analytical techniques is crucial for anti-doping efforts.

Purpose of the Study:

  • To develop a capillary-driven microfluidic chip-based immunoassay for sensitive and selective detection of hCG in urine.
  • To utilize antibody-modified magnetic metal-organic framework nanoparticles (MMOFs) for efficient hCG capture.
  • To establish a Surface-Enhanced Raman Spectroscopy (SERS) based detection system for quantifying captured hCG.

Main Methods:

  • Fabrication of a microfluidic chip with four chambers for sequential capture and detection.
  • Immobilization of anti-hCG antibodies onto magnetic metal-organic framework nanoparticles (MMOFs) for sample enrichment.
  • Utilizing gold nanorods labeled with 5,5'-Dithiobis-(2-nitrobenzoic acid) (DTNB) as SERS tags.
  • Employing a magnetic field for MMOF manipulation within the microfluidic chip.
  • Quantification of hCG via SERS signal measurement of DTNB in the final chamber.

Main Results:

  • The developed method demonstrated high selectivity, distinguishing hCG from other proteins like hLH, hGH, and IgG.
  • A regression coefficient of 0.9985 and a limit of detection of 0.61 IU/L were achieved.
  • Results showed high agreement (97.9% similarity) with the conventional ELISA method.
  • The system successfully detected hCG in urine samples.

Conclusions:

  • The capillary-driven microfluidic chip immunoassay provides a sensitive, selective, and rapid method for hCG detection.
  • This technique offers a viable and cost-effective alternative to traditional methods like Western Blot and ELISA for anti-doping applications.
  • The developed platform holds potential for routine analysis of prohibited substances in sports.