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Genotyping-free Selection of Double Allelic Gene Edited Medaka Using Two Different Fluorescent Proteins
Yu Murakami1, Satoshi Ansai1,2, Akari Yonemura1
1Division of Applied Bioscience, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
Bio-Protocol
|October 1, 2021
Abstract:
This protocol describes a simple genotyping using two different colors of fluorescent protein genes inserted at the target locus. This method makes it possible to determine the genotype of each individual simply by observing the fluorescence later than F1 generation.

