Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The DNA Helix

The DNA Helix

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multifactorial Risk Assessment and Anticoagulation Strategy Optimization for Deep Vein Thrombosis After Major Joint Surgery: A Retrospective Study.

Journal of visualized experiments : JoVE·2026

Same author

Corrigendum to 'A new perspective on targeting pulmonary arterial hypertension: Programmed cell death pathways (Autophagy, Pyroptosis, Ferroptosis)' [Biomed. Pharmacother. 181 (2024) 117706].

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026

Same author

FeS<sub>2</sub>/CuFeS<sub>2</sub> Composite Anodes Based on Seafloor Massive Sulfides Compositions for Lithium-Ion Batteries.

Materials (Basel, Switzerland)·2026

Same author

Functional Interpretation of Recurrent Genetic Variants in Hepatocellular Carcinoma: Molecular Consequences and Clinical Relevance.

Human mutation·2026

Same author

Molecular mechanisms of iron metabolism and ferroptosis in cardiovascular diseases and intervention strategies targeting natural products (Review).

Molecular medicine reports·2026

Same author

Short-chain acyl-CoA dehydrogenase: a novel and promising therapeutic target for heart failure via modulation of mitochondrial fatty acid oxidation.

Journal of hypertension·2026

Search research articles

Related Experiment Video

Updated: Oct 18, 2025

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Reconfigurable Two-Dimensional DNA Lattices: Static and Dynamic Angle Control.

Wen Wang¹, Chunyu Chen¹, Simon Vecchioni²

¹School of Life Sciences, Tsinghua University-Peking University Center for Life Sciences, Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China.

Angewandte Chemie (International Ed. in English)

|October 1, 2021

Summary

This summary is machine-generated.

Researchers developed new methods to control DNA branching orientation for synthetic DNA nanostructures. This advancement enables precise structural ordering and the creation of novel DNA nanomechanical devices.

Keywords:

DNA nanostructures angle control branching orientation junction motif self-assembly

More Related Videos

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Related Experiment Videos

Last Updated: Oct 18, 2025

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

Area of Science:

Synthetic biology
Nanotechnology
Biochemistry

Background:

Branched DNA motifs are fundamental to synthetic DNA nanostructures.
Controlling branching orientation is crucial for enhancing structural order and complexity.

Purpose of the Study:

To present novel strategies for precise control over DNA branching orientation.
To enable the development of advanced DNA nanostructures and nanomechanical devices.

Main Methods:

Utilizing immobile Holliday junctions with specific nucleotide sequences to dictate orientation.
Employing angle-enforcing struts with flexible spacers to fix branching angles.
Investigating dynamic orientation control via Watson-Crick base pairing and non-canonical nucleobase interactions (i-motif, G-quadruplex).

Main Results:

Demonstrated two distinct strategies for controlling DNA branching orientation.
Showcased dynamic control of branching orientation through various base-pairing mechanisms.
Established precise angle control and environmental feedback for DNA nanostructures.

Conclusions:

Precise control of DNA branching orientation is achievable through sequence-specific junctions and structural elements.
Dynamic control mechanisms offer versatility for responsive DNA nanostructures.
These advancements pave the way for sophisticated DNA nanomechanical sensors and ordered 3D architectures.